Latest revision as of 12:55, 21 October 2015

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

July
August
September

July 27th

Beginning of the manufacturing project! Let's do it!

Goal

The goal of this project is to create an way to grow and distribute our strains in India, easily and for cheap.
Since our genetically engineered strains will be ready only in september, we will not have time to work on it, so we have to find similar strains, that we can study and select with antibiotics.
We chose Saccharomyces cerevisiae mcherry, wich is resistant to geneticin at a concentration of 200µg/mL, and produce RFP. Therefore, it's easy to select and characterize it.
Basically, we are going to try different ways to distribute the strains and see how well they survived for each process.
In order to do that, we will have to calculate a survival rate so we have to be able to know how many cells we are putting in the beggining, in the recipe we are trying.

Material

Glycerol stock of Sc. mcherry
YPD broth
Agar
Geneticin, 100mg/ml
Osmosed water
Sterile culture tubes
Sterile eppendorf tubes
Sterile plates and beads

Procedure

Make a culture of Sc. mcherry in 10ml of YPD media adding 20µL of geneticin
Two days later, centrifuge the culture tube
Throw the media away and replace it by 10ml of osmosed water
Measure the OD, doing the blank with osmosed water
If the OD is too high to be measured, make a 10 times dilution and measure the OD of the dilution
In the eppendorf tubes, make 10^-1 to 10^-5 dilutions of the yeasts/water solution to plate them on YPDagar+GEN(200)

That is what we did today, and we plated the different dilutions, thanks to the beads.

July 28th

Goal

Our first idea to distribute the yeast was to simply dry them and collect the dry cells, without adding anything.
Today is a first try, to have an idea of how to do it.

Material

Culture of Sc. mcherry
YPD broth
Agar
Geneticin, 100mg/ml
Osmosed water
Sterile eppendorf tubes
Sterile plates and beads

Procedure

Centrifuge a culture tube
Throw the media away
Add 300µL of water to detach the yeasts from the tube easily
Spread on aluminium
Let it dry for 2 hours in the open air or in the incubator (2 different tests)
Scratch the aluminium to detach the dried yeasts
Put the powder in 10ml of osmosed water
Plate the solution to see if Sc. mcherry survived

Results and discussion

We obtained this powder:

We realised the aluminium was not a good idea:
The yeasts stick to it and it gets destroyed when we scratch.
We have to find another drying paper, more resistant.

July 29th

Result of the OD test made on the 27th of July:

The 10ml solution of Sc. mcherry/water had an OD of 2,557.
100µL of the 10^-4 dilution grew 359 colonies, so:
1 A = 1,4x10⁷ cells/ml
We repeated the very same OD experiment today, for more repetitions.

July 30th

Results of the drying test made on the 28th of July:

Yeasts grew in the plate.
We can check that they produce RFP, it's Sc. mcherry who survived.

Procedure

Today, we are repeating the drying experiment (see July 28th) but drying the yeasts on a plastic dish.

Take 100µL of the culture solution of Sc. mcherry to make dilutions (10^-1 to 10^-5)
Plate 100µL of each dilutions to deduce the number of cells in the culture tube
Centrifuge the culture tube
Throw the media away
Add 300µL of water to detach the yeasts from the tube easily
Spread on aluminium
Let it dry for 2 hours
Scratch the aluminium to detach the dried yeasts
Put the powder in 10ml of osmosed water
Take 100µL of the solution to make dilutions (10^-1 to 10^-5)
Thanks to the beads, plate 100µL of each dilution

July 31th

Result of the OD test made on the 29th of July:

The 10ml solution of Sc. mcherry/water had an OD of 2,635.
100µL of the 10^-4 dilution grew 205 colonies, so:
1 A = 7,8x10⁶ cells/ml

August 1st

Result of the drying test made on the 30th of July:

100µL of the 10^-5 dilution of the initial 10ml culture solution grew 106 colonies, so we initially dried 1,06x10⁹ cells.
100µL of the 10^-4 dilution of the powder mixed with 30ml of osmosed water grew 123 colonies, so 8,4x10⁸ cells survived.
We therefore have a survival rate of 79%.

Discussion and New Goal

The powder we obtained with the drying method stick to everything, because of static electricity and therefore, it is not very easy to distribute and manipulate.
Consequently, we are looking fo other solutions...

Our second idea was to cook a homemade powder with edible ingredients easily found in India.
We found a recipe that inspired us here.
We decided to compare the initial recipe to our modifications, so we made for different tests :

Test 1, original recipe: wheat flour, sugar and ginger
Test 2: rice flour, sugar and ginger
Test 3: rice flour, sugar
Test 4: only rice flour

Material

Culture of Sc. mcherry
Potatoes
Flour (wheat and rice)
Cornmeal
Sugar
Ginger
Plate of YPDagar+Gen(200)
Osmosed water
Sterile eppendorf tubes
Sterile beads

Procedure

Cook some potatoes
Save the cooking water, wait till it's not too hot and mix 20ml of it with the yeasts
Add 20ml of flour (rice or wheat depending on the recipe), 40ml of mashed potatoes
Depending on the recipe add 20ml of sugar and a teaspoon of ginger
Add 80ml of cornmeal
Let it dry for 1 day
Mill the paste to obtain a powder
Plate the powder obtained to see how many Sc. mcherry survived

August 3rd

Continuing the powder recipe we began on the 1st of August

After almost two days of drying, we obtained a very hard paste that we milled.
After milling, we had this nice yellow powder you can see on the picture.

We put 100mg of each powder in 10ml of osmosed water, made dilutions,
and plated the solutions in YPDagar+GEN(200) plates.

August 4th

Discussion and New Goal

While doing the recipe with the potatoes (from the 1st to the 3rd of August), we realised it wasn't easy to do and it took lots of time and ingredients.
We decided to reduce the number of ingredients, using only water and rice flour.

Procedure

Mix the yeasts with 20ml of osmosed water
Add 40ml of rice flour
Knead till you get a nice paste

At this point, we tought the paste could be shaped, instead of being milled in a powder.

Shape it in little cubes
Let it dry for few hours

Result

The result is a small solid cube, very easy to give away.

Several cubes can be packed together.

We tought it could be a good media of distribution.

We dissolved one cube in 10ml of osmosed water, after weighed it.
Then we made dilutions (10^-1 to 10^-5) and plated 100µL of each on YPDagar+GEN(200)

August 5th

Results of the survival of Sc. mcherry in the potato powder made on the 3rd of August:

Survival rate:

Wheat flour, sugar and ginger: 14%
Rice flour, sugar and ginger: 20%
Rice flour, sugar: 12%
Rice flour: 4%

Discussion:

We can see that the more ingredients we put, the more the yeasts survived, so each ingredient plays a role in the cells survival.
But as we said before, we don't want to put too much ingredients in the recipe so that it's cheap and easy.
We can also notice that rice flour seems more efficient than wheat flour (but we made only one repetition so nothing is statistically proven).

August 6th

Results of the survival of Sc. mcherry in the cubes made on the 4th of August:

We obtained a survival rate of 69%.

After staying 5hours in the water, the survival rate reached 737%.

August 7th

Goal

Now that we are at ease with the process, we are trying the very same process with lactococcus lactis. We chose MG 13.63, containing the plasmid PSIP411, resistant to erythromycin at a concentration of 10µL/mL, to study it's survival rate.
We will name this strain G1513.
We also have to correlate the OD of a solution of G1513 to the number of alive cells inside.

Material

Glycerol stock of G1513
M17 broth
Solution of D-Glucose
Agar
Erythromycin, 20mg/ml
Osmosed water
Sterile eppendorf tubes
Sterile plates and beads

Procedure

Make a culture of G1513 in 10ml of M17+1% glucose media adding 5µL of erythromycin
One day later, centrifuge the culture tube
Throw the media away and replace it by 10ml of osmosed water
Measure the OD, doing the blank with osmosed water
If the OD is too high to be measured, make a 10 times dilution and measure the OD of the dilution
In the eppendorf tubes, make 10^-1 to 10^-5 dilutions of the G1513/water solution to plate 100µL of each on M17Glucose+agar+Ery(10)

August 10th

Results of the OD test for G1513 made on the 7th of August:

The 10ml solution of G1513/water had an OD of 1,296.
Here is a table of what 100µL of each dilutions grew:

Dilution	Number of colonies
10^-1	381
10^-2	84
10^-3	11

We have, in average, 7,74x10³ cells per ml of the 10^-1 dilution.
so: 1 A = 6,0x10⁴ cells/ml

Procedure

Today, we made new cubes containing Sc. mcherry and G1513 to calculate the survival rate.

August 11th

Goal

While waiting for the cubes results, we are thinking about the next step: finding a media to grow the strains, easy to give to the population and to use afterward.
The perfect media would be an edible one, that we could incorporate to the cube, instead of the water.

Material

Culture of Sc. mcherry
3 potatoes
Rice flour
Geneticin, 100mg/ml
Osmosed water
Sterile eppendorf tubes and culture tubes
Sterile plates and beads
One glass bottle

Procedure

We tried 2 different medium: potato juice and potato juice with rice flour.

For the potato juice:

Heat 3 potatoes in 750ml of water
Let the water boil (sterilisation)
Save the water and stock it in a bottle
Put 10ml of this potato water in a culture tube
Add 20μL of geneticin (100mg/ml)
Inoculate with Sc. mcherry
Put in the incubator at 30°C for 2 days

For the mix potato juice with rice flour:

Put 5ml of the potato water made previously in a culture tube
Inoculate with Sc. mcherry
Add 20μL of geneticin (100mg/ml)
Add 5ml of rice flour
Put in the incubator at 30°C for 2 days

August 12th

Results of the cube tests made on the 10th of August

The yeasts survived well: 42%.
(Seven days later, on the 19th of August, we plated cubes from this same test, and obtained a survival rate of 21%.)
But about the G1513, strangely, the number of cells in the plates of M17agar, which are supposed to be G1513, is the same as in the plates with YPD and geneticin, selecting the yeasts.
Therefore, we checked the production of RFP, since Sc. mcherry produces RFP but not G1513.
Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. mcherry resist to erythromycin and is therefore growing in the plate.

Goal

We need a chemical that will allow us to select G1513 and kill Sc. mcherry.
After some researchs, we found out that cycloheximide could be helpful and ordered it. While waiting for this chemical, we can't work with G1513.

August 13th

Results of the media test made on the 11th of August

Nothing grew on the potato juice media.
We had the idea of adding sugar, to see if it changes something.
The potato juice/rice flour media strongly smells yeasts. We therefore admit the yeast grew in this media but decided that the aspect wasn't very attractive and the smell so so bad that indian population would be disgusted and won't use it.

August 17th

Procedure

Making new cubes with Sc. mcherry alone, G1513 alone, and a mix of both strains.
Measuring the OD and plating to count the cells

Results of the media test made on the 13th of August

With sugar, we can see there are cells growing in the potato juice.
It's a very small growth compared to the one in YPD but it's still a growth.
We will work more precisely on this media, trying to deduce the influence of the potato and sugar concentration.

August 18th

Results of the OD test made on the 17th of August

For Sc. mcherry:
The 10ml solution of Sc. mcherry/water had an OD of 1,820.
100µL of the 10^-4 dilution grew 70 colonies, so:
1 A = 3,8x10⁶ cells/ml
For G1513:
The 10ml solution of G1513/water had an OD of 1,521.
100µL of the dilutions grew:

Dilution	Number of colonies
10^-5	131
10^-6	21

So 1 A = 1,1x10⁸ cells/ml

August 19th

Results of the cube test made on the 17th of August

Sc. mcherry alone: 39%
Sc. mcherry in the mix: 26%
G1513 in the mix: not plated because we are waiting for the cycloheximide
G1513 alone: 1,8%

We kept samples of the mix in the fridge to be able to study it later, when we will have the cycloheximide.

August 20th

Procedure

We made more cubes with Sc. mcherry alone, G1513 alone, and a mix of both.
We made more OD correlations.

August 24th

Procedure

We made more cubes...

Results of the OD test made on the 20th of August

For Sc. mcherry:
The 10ml solution of Sc. mcherry/water had an OD of 1,828.
100µL of the 10^-5 dilution grew 56 colonies, so:
1 A = 3,1x10⁶ cells/ml
For G1513:
Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.

Results of the cube test made on the 20th of August

Sc. mcherry alone: 92%
Sc. mcherry in the mix (but dead G1513): 96%
G1513 alone: Nothing

August 25th

Results of the OD tests made on the 24th of August

For Sc. mcherry:
The first 10ml solution of Sc. mcherry/water had an OD of 1,921.
100µL of the dilutions grew:

Dilution	Number of colonies
10^-4	505
10^-5	39

1 A = 2,3x10⁶ cells/mL
The second 10ml solution of Sc. mcherry/water had an OD of 2,523.
100µL of the 10^-5 dilution grew 95 colonies.
so 1 A = 3,8x10⁶ cells/mL
For G1513:
Nothing... We used cells from the previous culture, wich was dead, so it seems normal.

August 26th

Results of the cube test made on the 24th of August

Sc. mcherry alone: 122% et 162%

Sc. mcherry in the mix (but dead G1513): 178% et 217%

G1513 alone: Nothing

August 27th

Goal

We want to find a media that could be use in India to grow the yeasts and the Lactococcus lactis for cheap.

Material

Potatoes
Sugar
Water
Culture of Sc. mcherry
Culture of G1513
Erythromycin, Geneticin
Tecan and Tecan plate
Mineral oil

Procedure

Make 3 different potato juice solutions(low, medium and high concentration of potatoes) following this protocol
For each one of these solutions, make samples with different concentration of sugar: 0g/ml; 0,1g/ml; 0,2g/ml; 0,4g/ml.
Inoculate the strains in these media, with the appropriate antibiotics (do not mix G1513 and Sc. mcherry)
Put 150μL of the solutions in Tecan plate wells and add 50μL of oil
Put in the Tecan at 30°C, measuring the OD every 15minutes, for 2 days

August 28th

Goal

We want to see how the strains in the cubes react when put in idli.

Procedure

Following the idli recipe protocol, make idli.
Weigh the cube
Dilute the cube in 5ml of osmosed water
Add this solution to 1 bottle of idli before the fermentation
After the fermentation, plate 100μL of a 10^-2 dilution of the Idli water

August 31th

Results of the media test made on the 27th of August

It's a fail, the OD was too high so we can't really see the growth.
We did it again, diluting the inoculated media 200times in osmosed water.

Results of the idli test

	Number of cells in the top part of the idli, 10^-2 dilution	Number of cells in the middle part of the idli, 10^-2 dilution	Number of cells in the bottom part of the idli, 10^-2 dilution
X	38 / 23	324	71 / 111
Y	0	39 / 27	64 / 47

September 2nd

Results of the second media test made on the 31st of August

It's a fail, there was almost only water in the well so nothing grew.
We will do it again, asking for a real protocol to our advisors, because obviously, there was a misunderstanding.

September 4th

Procedure

We tried the new method for the Tecan test.
This time, we did an overnight culture for each media and each strain, that we diluted 200times (till we get an OD smaller than 0,01)

September 6th

Procedure

We made fresh new cultures of G1513 and Sc. mcherry.

September 7th

Results of the Tecan test made on the 4th of September

Third fail, because one parameter was changed and we didn't noticed: the Tecan measured the OD for only few hours, not enough to see the growth...

September 8th

Procedure

Today we used the fresh cultures we made on the 6th of August to make lots of new cubes and OD test.
After centrifugation, throwing the media and replacing it by osmosed water, the cultures gave us two solutions:

Solution X: 8,8x10⁷ cells of Sc. mcherry per ml
Solution Y: 5,2x10⁹ cells of G1513 per ml

We made 3 different types of cubes:

Cubes Q: 15ml of rice flour, 6ml of osmosed water and 1ml of solution X
Cubes R: 15ml of rice flour, 5ml of osmosed water, 900μL of solution X and 1ml of solution Y
Cubes S: 15ml of rice flour, 6ml of osmosed water and 1ml of solution Y

Strains and cubes	Total weight of the cubes (g)	Number of cells per g of cube
Sc. mcherry in Q cubes	13,30	6,6x10⁶
Sc. mcherry in R cubes	13,39	5,9x10⁶
G1513 in R cubes	13,39	3,9x10⁸
Sc. mcherry in S cubes	10,70	4,8x10⁸

We are going to plate one cube of each kind each day and follow the survival rate during a week of drying. For the OD, here are the results we obtained (we obtained them on the 10th of August).

Studied strains	OD of the sample	Number of colonies in the 10^-3 dilution of the sample	Number of colonies in the 10^-4 dilution of the sample	Number of colonies in the 10^-5 dilution of the sample
Sc. mcherry	1,156	+++	347	23
G1513	0,392	+++	439	/

So, for Sc. mcherry: 1A=2,5x10⁷ cells/ml
For G1513: 1A=1,1x10⁸ cells/ml

September 9th

We plated the first cubes today.
Everyday, we will follow the same protocol:

Weigh the cube
Dilute the cube in 10ml of osmosed water during 5min
Dilute the solution obtained 10^-1, 10^-2 and 10^-3 times
Plate the dilutions

This should only take 20min in total, doing it for all the cubes in the same time.
For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating).

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-3 dilution	Number of colonies in the 10^-4 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	0,96	6,3x10⁶	72	10	8,6x10⁶	136,5%
Sc. mcherry in R	0,96	5,7x10⁶	80	11	9,5x10⁶	166,7%
G1513 in R	0,96	3,7x10⁸	79	10	9,0x10⁶	2,4%
G1513 in S	0,72	5,4x10⁸	357	23	2,9x10⁷	5,4%

September 10th

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-3 dilution	Number of colonies in the 10^-4 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	0,97	6,4x10⁶	104	5	7,7x10⁶	120,3
Sc. mcherry in R	1,40	8,3x10⁶	150	11	1,3x10⁷	156,6
G1513 in R	1,40	5,5x10⁸	126	/	1,3x10⁷	2,4
G1513 in S	1,19	5,7x10⁸	63	/	6,3x10⁶	1,1%

September 11th

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-2 dilution	Number of colonies in the 10^-3 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	1,04	6,9x10⁶		77	7,7x10⁶	111,5%
Sc. mcherry in R	1,28	8,5x10⁶		149	1,5x10⁷	176,5
G1513 in R	1,28	5,0x10⁸	164	/	1,6x10⁶	0,3%
G1513 in S	1,49	5,8x10⁸	299	/	3,0x10⁶	0,5%

September 12th

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-2 dilution	Number of colonies in the 10^-3 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	1,50	9,9x10⁶	877	88	1,3x10⁷	131,3%
Sc. mcherry in R	1,12	6,6x10⁶	513	52	7,2x10⁶	109,1%
G1513 in R	1,12	4,4x10⁸	331	/	3,3x10⁶	0,8%
G1513 in S	0,93	4,5x10⁸	368	/	3,7x10⁶	0,8%

September 13th

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-2 dilution	Number of colonies in the 10^-3 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	1,17	7,7x10⁶	/	123	1,2x10⁷	155,8%
Sc. mcherry in R	1,50	8,9x10⁶	/	117	1,2x10⁷	134,8%
G1513 in R	1,50	5,9x10⁸	28	/	2,8x10⁵	0,05%
G1513 in S	0,96	4,6x10⁸	30	/	3,0x10⁵	0,07%

September 14th

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-2 dilution	Number of colonies in the 10^-3 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	1,18	7,8x10⁶	/	103	1,0x10⁷	128,2%
Sc. mcherry in R	1,33	7,9x10⁶	/	83	8,3x10⁶	105,1%
G1513 in R	1,33	5,2x10⁸	12	/	1,2x10⁵	0,02%
G1513 in S	0,82	3,9x10⁸	49	/	4,9x10⁵	0,1%

September 15th

Studied strains	Weight (g)	Number of cells initially in the cube	Number of colonies in the 10^-2 dilution	Number of colonies in the 10^-3 dilution	Number of cells in the cube after the drying period	Survival rate
Sc. mcherry in Q	1,08	7,1x10⁶	/	91	9,1x10⁶	128,2
Sc. mcherry in R	1,44	8,5x10⁶	/	84	8,4x10⁶	98,8
G1513 in R	1,44	5,6x10⁸	4	/	4,0x10⁴	0,0%
G1513 in S	1,01	4,9x10⁸	10	/	1,0x10⁵	0,0%

@@ Line 1: / Line 1: @@
 {{Paris_Bettencourt/header}}
 {{Paris_Bettencourt/menu}}
-{{Paris_Bettencourt/notebookBanner}}
+{{Paris_Bettencourt/banner|page_id=notebook|page_name=Notebook - Manufacturing}}
 <html>
@@ Line 20: / Line 20: @@
 The goal of this project is to create an way to grow and distribute our strains in India, easily and for cheap.<br>
 Since our genetically engineered strains will be ready only in september, we will not have time to work on it, so we have to find similar strains, that we can study and select with antibiotics.<br>
-We chose Saccharomyces cerevisiae  mcherry, wich is resistant to geneticin at a concentration of 200µg/mL, and produce RFP.<br>
+We chose Saccharomyces cerevisiae  mcherry, wich is resistant to geneticin at a concentration of 200µg/mL, and produce RFP. Therefore, it's easy to select and characterize it.<br>
 Basically, we are going to try different ways to distribute the strains and see how well they survived for each process.<br>
 In order to do that, we will have to calculate a survival rate so we have to be able to know how many cells we are putting in the beggining, in the recipe we are trying.
@@ Line 44: / Line 44: @@
 <li>Measure the OD, doing the blank with osmosed water</li>
 <li>If the OD is too high to be measured, make a 10 times dilution and measure the OD of the dilution</li>
-<li>In the eppendorf tubes, make 10^-1 to 10^-5 dilutions of the yeasts/water solution to plate them on YPDagar+GEN(200)</li></ul>
+<li>In the eppendorf tubes, make 10<sup>-1</sup> to 10<sup>-5</sup> dilutions of the yeasts/water solution to plate them on YPDagar+GEN(200)</li></ul>
 <br>
 That is what we did today, and we plated the different dilutions, thanks to the beads.
@@ Line 96: / Line 96: @@
 <H2>Result of the OD test made on the 27th of July:</h2>
 The 10ml solution of Sc. mcherry/water had an OD of 2,557.<br>
-µL of the 10^-4 dilution grew 359 colonies, so:<br>
+µL of the 10<sup>-4</sup> dilution grew 359 colonies, so:<br>
-<b>1 OD = 1,4x10^7 cells/ml</b><br>
+<b>1 A = 1,4x10<sup>7</sup> cells/ml</b><br>
 We repeated the very same OD experiment today, for more repetitions.
@@ Line 110: / Line 110: @@
 <h2>Procedure</h2>
 Today, we are repeating the drying experiment (see July 28th) but drying the yeasts on a plastic dish.<br>
-<ul><li>Take 100µL of the culture solution of Sc. mcherry to make dilutions (10^-1 to 10^-5)</li>
+<ul><li>Take 100µL of the culture solution of Sc. mcherry to make dilutions (10<sup>-1</sup> to 10<sup>-5</sup>)</li>
 We remind that the culture tube contain 10ml of media.
 <li>Plate 100µL of each dilutions to deduce the number of cells in the culture tube</li>
@@ Line 120: / Line 120: @@
 <li>Scratch the aluminium to detach the dried yeasts</li>
 <li>Put the powder in 10ml of osmosed water</li>
-<li>Take 100µL of the solution to make dilutions (10^-1 to 10^-5)</li>
+<li>Take 100µL of the solution to make dilutions (10<sup>-1</sup> to 10<sup>-5</sup>)</li>
 <li>Thanks to the beads, plate 100µL of each dilution</li></ul>
@@ Line 128: / Line 128: @@
 <h2>Result of the OD test made on the 29th of July:</h2>
 The 10ml solution of Sc. mcherry/water had an OD of 2,635.<br>
-µL of the 10^-4 dilution grew 205 colonies, so:<br>
+µL of the 10<sup>-4</sup> dilution grew 205 colonies, so:<br>
-<b>1 OD = 7,8x10^6 cells/ml</b>
+<b>1 A = 7,8x10<sup>6</sup> cells/ml</b>
-<br><br>
+<br>
+<a name="august" class="anchor"><h1></h1></a>
 <h1 class="date two">August 1st</h1>
 <h2>Result of the drying test made on the 30th of July:</h2>
-µL of the 10^-5 dilution of the initial 10ml culture solution grew 106 colonies, so we initially dried 1,06x10^9 cells.<br>
+µL of the 10<sup>-5</sup> dilution of the initial 10ml culture solution grew 106 colonies, so we initially dried 1,06x10<sup>9</sup> cells.<br>
-µL of the 10^-4 dilution of the powder mixed with 30ml of osmosed water grew 123 colonies, so 8,4x10^8 cells survived.<br>
+µL of the 10<sup>-4</sup> dilution of the powder mixed with 30ml of osmosed water grew 123 colonies, so 8,4x10<sup>8</sup> cells survived.<br>
 We therefore have a survival rate of 79%.
@@ Line 144: / Line 145: @@
 <br>
 Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br>
-We found a recipe that inspired us here: http://www.instructables.com/id/Stop-Paying-for-Yeast-Make-Your-Own/<br>
+We found a recipe that inspired us <a href="http://www.instructables.com/id/Stop-Paying-for-Yeast-Make-Your-Own/">here</a>. <br>
 We decided to compare the initial recipe to our modifications, so we made for different tests :<br>
 <ul>
@@ Line 190: / Line 191: @@
 <br>
 <h1 class="date two">August 3rd</h1>
-<h2>Continuing the experiment we began on the 1st of August</h2>
+<h2>Continuing the powder recipe we began on the 1st of August</h2>
 <div class="column-left"><p>
 <br>
@@ Line 228: / Line 229: @@
 We tought it could be a good media of distribution.<br><br>
 We dissolved one cube in 10ml of osmosed water, after weighed it.<br>
-Then we made dilutions (10^-1 to 10^-5) and plated 100µL of each on YPDagar+GEN(200)
+Then we made dilutions (10<sup>-1</sup> to 10<sup>-5</sup>) and plated 100µL of each on YPDagar+GEN(200)
 </p></div>
 <div class="column-right"><p>
@@ Line 238: / Line 239: @@
 <br>
 <h1 class="date two">August 5th</h1>
-<h2>Results of the potato powder made on the 3rd of August:</h2>
+<h2>Results of the survival of Sc. mcherry in the potato powder made on the 3rd of August:</h2>
 Survival rate:
 <ul>
-<li>Wheat flour, sugar and ginger: 6,9% </li>
+<li>Wheat flour, sugar and ginger: 14% </li>
-<li>Rice flour, sugar and ginger: 9,5%</li>
+<li>Rice flour, sugar and ginger: 20%</li>
-<li>Rice flour, sugar: 5,7%</li>
+<li>Rice flour, sugar: 12%</li>
-<li>Rice flour: 1,9%</li>
+<li>Rice flour: 4%</li>
 </ul>
 <h2>Discussion:</h2>
@@ Line 253: / Line 254: @@
 <br><br>
 <h1 class="date two">August 6th</h1>
-<h2>Results of the cube test made on the 4th of August:</h2>
+<h2>Results of the survival of Sc. mcherry in the cubes made on the 4th of August:</h2>
 <div class="column-left"><p>
 <br>
-We obtained a survival rate of 6,9%. <br><br>
+We obtained a survival rate of 69%. <br><br>
 After staying 5hours in the water, the survival rate reached 737%.
 </p></div>
@@ Line 291: / Line 292: @@
 <li>Measure the OD, doing the blank with osmosed water</li>
 <li>If the OD is too high to be measured, make a 10 times dilution and measure the OD of the dilution</li>
-<li>In the eppendorf tubes, make 10^-1 to 10^-5 dilutions of the G1513/water solution to plate 100µL of each on M17Glucose+agar+Ery(10)</li></ul>
+<li>In the eppendorf tubes, make 10<sup>-1</sup> to 10<sup>-5</sup> dilutions of the G1513/water solution to plate 100µL of each on M17Glucose+agar+Ery(10)</li></ul>
 <br>
@@ Line 304: / Line 305: @@
    </tr>
    <tr align="center">
-     <td>10^-1</td>
+     <td>10<sup>-1</sup></td>
      <td>381</td>
    </tr>
    <tr align="center">
-     <td>10^-2</td>
+     <td>10<sup>-2</sup></td>
      <td>84</td>
    </tr>
    <tr align="center">
-     <td>10^-3</td>
+     <td>10<sup>-3</sup></td>
      <td>11</td>
    </tr>
 </table>
-We have, in average, 774 cells in 100µL of the 10^-1 dilution.<br>
+We have, in average, 7,74x10<sup>3</sup> cells per ml of the 10<sup>-1</sup> dilution.<br>
-so: <b>1 OD = 6,0x10^4 cells/ml</b><br>
+so: <b>1 A = 6,0x10<sup>4</sup> cells/ml</b><br>
 <h2>Procedure</h2>
-Tody, we made new cubes containing Sc. mcherry and G1513 to calculate the survival rate.
+Today, we made new cubes containing Sc. mcherry and G1513 to calculate the survival rate.
 <br><br>
@@ Line 336: / Line 337: @@
 <li>Osmosed water</li>
 <li>Sterile eppendorf tubes and culture tubes</li>
-<li>Sterile plates and beads</li></ul>
+<li>Sterile plates and beads</li>
-<li>One glass bottle</li>
+<li>One glass bottle</li></ul>
 <h2>Procedure</h2>
@@ Line 362: / Line 363: @@
 <br>
 <h1 class="date two">August 12th</h1>
-<h2>Results of the cube tests</h2>
+<h2>Results of the cube tests made on the 10th of August</h2>
 The yeasts survived well: 42%. <br>
-Seven days later, we plated again cubes from this test, and obtained a survival rate of 21%.<br>
+(Seven days later, on the 19th of August, we plated cubes from this same test, and obtained a survival rate of 21%.)<br>
-But strangely, the number of cells in the plates of M17 glucose with erythromycin, which are supposed to be G1513,  is the same as in the plates with YPD and geneticin, selecting the yeasts.<br>
+But about the G1513, strangely, the number of cells in the plates of M17agar, which are supposed to be G1513,  is the same as in the plates with YPD and geneticin, selecting the yeasts.<br>
 Therefore, we checked the production of RFP, since Sc. mcherry produces RFP but not G1513.<br>
 Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. mcherry resist to erythromycin and is therefore growing in the plate.<br>
 <h2>Goal</h2>
 We need a chemical that will allow us to select G1513 and kill Sc. mcherry.<br>
@@ Line 374: / Line 376: @@
 <br><br>
 <h1 class="date two">August 13th</h1>
-<h2>Results of the media test</h2>
+<h2>Results of the media test made on the 11th of August</h2>
 Nothing grew on the potato juice media.<br>
 We had the idea of adding sugar, to see if it changes something.<br>
@@ Line 385: / Line 387: @@
 Measuring the OD and plating to count the cells
-<h2>Results of the media test</h2>
+<h2>Results of the media test made on the 13th of August</h2>
 With sugar, we can see there are cells growing in the potato juice.<br>
 It's a very small growth compared to the one in YPD but it's still a growth.<br>
-We will work more precisely on this media, trying to deduce the influence of the concentration in potato and in sugar.
+We will work more precisely on this media, trying to deduce the influence of the potato and sugar concentration.
 <br><br>
 <h1 class="date two">August 18th</h1>
-<h2>Results of the OD test</h2>
+<h2>Results of the OD test made on the 17th of August</h2>
 For Sc. mcherry:<br>
-<b>1 OD = 3,8x10^6 cells/ml</b><br>
+The 10ml solution of Sc. mcherry/water had an OD of 1,820.<br>
+µL of the 10<sup>-4</sup> dilution grew 70 colonies, so:<br>
+<b>1 A = 3,8x10<sup>6</sup> cells/ml</b><br>
 For G1513:<br>
-<b>1 OD = 1,1x10^8 cells/ml</b>
+The 10ml solution of G1513/water had an OD of 1,521.<br>
+µL of the dilutions grew:<br>
+<table style="width:25%" align="center">
+  <tr style="background-color:#E6E6E6">
+    <th>Dilution </th>
+    <th>Number of colonies</th>
+  </tr>
+  <tr align="center">
+    <td>10<sup>-5</sup></td>
+    <td>131</td>
+  </tr>
+  <tr align="center">
+    <td>10<sup>-6</sup></td>
+    <td>21</td>
+  </tr>
+</table>
+So <b>1 A = 1,1x10<sup>8</sup> cells/ml</b>
 <br><br>
 <h1 class="date two">August 19th</h1>
-<h2>Results of the cube test</h2>
+<h2>Results of the cube test made on the 17th of August</h2>
 <ul>
 <li>Sc. mcherry alone: 39%</li>
 <li>Sc. mcherry in the mix: 26%</li>
+<li>G1513 in the mix: not plated because we are waiting for the cycloheximide</li>
 <li>G1513 alone: 1,8%</li>
 </ul>
 We kept samples of the mix in the fridge to be able to study it later, when we will have the cycloheximide.
 <br><br>
 <h1 class="date two">August 20th</h1>
 <h2>Procedure</h2>
-Making more cubes with Sc. mcherry alone, G1513 alone, and a mix of both.<br>
+We made more cubes with Sc. mcherry alone, G1513 alone, and a mix of both.<br>
-Making more OD correlations.
+We made more OD correlations.
 <br><br>
 <h1 class="date two">August 24th</h1>
 <h2>Procedure</h2>
-Making more cubes...<br>
+We made more cubes...<br>
-<h2>Results of the OD test</h2>
+<h2>Results of the OD test made on the 20th of August</h2>
 For Sc. mcherry:<br>
-<b>1 OD = 3,1x10^6 cells/ml</b><br>
+The 10ml solution of Sc. mcherry/water had an OD of 1,828.<br>
+µL of the 10<sup>-5</sup> dilution grew 56 colonies, so:<br>
+<b>1 A = 3,1x10<sup>6</sup> cells/ml</b><br>
 For G1513:<br>
 Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br>
-<h2>Results of the cube test</h2>
+<h2>Results of the cube test made on the 20th of August</h2>
 <ul>
 <li>Sc. mcherry alone: 92%</li>
@@ Line 433: / Line 461: @@
 <br>
 <h1 class="date two">August 25th</h1>
-<h2>Results of the OD test</h2>
+<h2>Results of the OD tests made on the 24th of August</h2>
 For Sc. mcherry:<br>
-<b>1 OD = 2,6x10^6 cells/mL</b><br>
+The first 10ml solution of Sc. mcherry/water had an OD of 1,921.<br>
+µL of the dilutions grew:<br>
+<table style="width:25%" align="center">
+  <tr style="background-color:#E6E6E6">
+    <th>Dilution </th>
+    <th>Number of colonies</th>
+  </tr>
+  <tr align="center">
+    <td>10<sup>-4</sup></td>
+    <td>505</td>
+  </tr>
+  <tr align="center">
+    <td>10<sup>-5</sup></td>
+    <td>39</td>
+  </tr>
+</table>
+<b>1 A = 2,3x10<sup>6</sup> cells/mL</b><br>
+The second 10ml solution of Sc. mcherry/water had an OD of 2,523.<br>
+µL of the 10<sup>-5</sup> dilution grew 95 colonies.<br>
+so <b>1 A = 3,8x10<sup>6</sup> cells/mL</b><br>
 For G1513:<br>
 Nothing... We used cells from the previous culture, wich was dead, so it seems normal.
 <br><br>
 <h1 class="date two">August 26th</h1>
-<h2>Results of the cube test</h2>
+<h2>Results of the cube test made on the 24th of August</h2>
 <p><img src="https://static.igem.org/mediawiki/2015/d/d9/PBmanufacturingcubeenmain.jpg" width="150" style="display:block; margin-right: 20px; float:left;">
 <li>Sc. mcherry alone: 122% et 162%</li><br>
@@ Line 452: / Line 500: @@
 <h2>Goal</h2>
 We want to find a media that could be use in India to grow the yeasts and the Lactococcus lactis for cheap.<br>
+<h2>Material</h2>
+<ul>
+<li>Potatoes</li>
+<li>Sugar</li>
+<li>Water</li>
+<li>Culture of Sc. mcherry</li>
+<li>Culture of G1513</li>
+<li>Erythromycin, Geneticin</li>
+<li>Tecan and Tecan plate</li>
+<li>Mineral oil</li>
+</ul>
 <h2>Procedure</h2>
 <ul>
-<li>Make 3 different potato juice solutions: low, medium and high concentration in potatoes</li>
+<li>Make 3 different potato juice solutions(low, medium and high concentration of potatoes) following this protocol</li>
-<li>For each one of these solutions, make samples with different concentration of sugar</li>
+<li>For each one of these solutions, make samples with different concentration of sugar: 0g/ml; 0,1g/ml; 0,2g/ml; 0,4g/ml.</li>
-<li>Inoculate the strains separetely in these media, with the appropriate antibiotics</li>
+<li>Inoculate the strains in these media, with the appropriate antibiotics (do not mix G1513 and Sc. mcherry)</li>
-<li>Put 150μL of the solutions in a Tecan plate well and add 50μL of oil</li>
+<li>Put 150μL of the solutions in Tecan plate wells and add 50μL of oil</li>
 <li>Put in the Tecan at 30°C, measuring the OD every 15minutes, for 2 days</li>
 </ul>
@@ Line 464: / Line 523: @@
 <h1 class="date two">August 28th</h1>
 <h2>Goal</h2>
-We want to see how the strains in the cubes react when put in the idli.<br>
+We want to see how the strains in the cubes react when put in idli.<br>
 <h2>Procedure</h2>
-???????????????
+<ul>
+<li>Following the <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#FSIdli" target="_blank">idli recipe protocol</a>, make idli.</li>
+<li>Weigh the cube</li>
+<li>Dilute the cube in 5ml of osmosed water</li>
+<li>Add this solution to 1 bottle of idli before the fermentation</li>
+<li>After the fermentation, plate 100μL of a 10<sup>-2</sup> dilution of the Idli water</li>
+</ul>
-<br><br>
+<br>
 <h1 class="date two">August 31th</h1>
-<h2>Results of the media test</h2>
+<h2>Results of the media test made on the 27th of August</h2>
 It's a fail, the OD was too high so we can't really see the growth.<br>
-We did it again, diluting the media and strain 200times in osmosed water.<br>
+We did it again, diluting the inoculated media 200times in osmosed water.<br>
 <h2>Results of the idli test</h2>
-????????????????????????????
+<table style="width:100%; text-align:center">
+ <tr>
+    <th></th>
+    <th>Number of cells in the top part of the idli, 10<sup>-2</sup> dilution</th>
+    <th>Number of cells in the middle part of the idli, 10<sup>-2</sup> dilution</th>
+    <th>Number of cells in the bottom part of the idli, 10<sup>-2</sup> dilution</th>
+  </tr>
+  <tr>
+    <th>X</th>
+    <td>38 / 23</td>
+    <td>324</td>
+    <td>71 / 111</td>
+  </tr>
+  <tr>
+    <th>Y</th>
+    <td>0</td>
+    <td>39 / 27</td>
+    <td>64 / 47</td>
+  </tr>
+</table>
+<br>
-<br><br>
+<a name="september" class="anchor"><h1 class="date three">September 2nd</h1></a>
-<h1 class="date three">September 2nd</h1>
+<h2>Results of the second media test made on the 31st of August</h2>
-<h2>Results of the second media test</h2>
 It's a fail, there was almost only water in the well so nothing grew.<br>
-We will do it again, asking for a real protocol to some experts.
+We will do it again, asking for a real protocol to our advisors, because obviously, there was a misunderstanding.
 <br><br>
 <h1 class="date three">September 4th</h1>
 <h2>Procedure</h2>
-Trying the new method for the Tecan test.<br>
+We tried the new method for the Tecan test.<br>
 This time, we did an overnight culture for each media and each strain, that we diluted 200times (till we get an OD smaller than 0,01)
+<br><br>
+<h1 class="date three">September 6th</h1>
+<h2>Procedure</h2>
+We made fresh new cultures of G1513 and Sc. mcherry.
 <br><br>
 <h1 class="date three">September 7th</h1>
-<h2>Results of the Tecan test</h2>
+<h2>Results of the Tecan test made on the 4th of September</h2>
 Third fail, because one parameter was changed and we didn't noticed: the Tecan measured the OD for only few hours, not enough to see the growth...
@@ Line 496: / Line 589: @@
 <h1 class="date three">September 8th</h1>
 <h2>Procedure</h2>
-We made fresh new culture of Sc. mcherry and G1513, that we used today to make lots of new cubes and OD test.
+Today we used the fresh cultures we made on the 6th of August to make lots of new cubes and OD test.<br>
-We planned on making many cubes to plate one each day and follow the survival rate during the week.
+After centrifugation, throwing the media and  replacing it by osmosed water, the cultures gave us two solutions:
+<ul><li>Solution X: 8,8x10<sup>7</sup> cells of Sc. mcherry per ml</li>
+<li>Solution Y: 5,2x10<sup>9</sup> cells of G1513 per ml</li></ul>
-<br><br>
+We made 3 different types of cubes:
+<ul>
+<li>Cubes Q: 15ml of rice flour, 6ml of osmosed water and 1ml of solution X</li>
+<li>Cubes R: 15ml of rice flour, 5ml of osmosed water, 900μL of solution X and 1ml of solution Y</li>
+<li>Cubes S: 15ml of rice flour, 6ml of osmosed water and 1ml of solution Y</li>
+</ul>
+<table style="width:100%; text-align:center">
+ <tr>
+    <th>Strains and cubes</th>
+    <th>Total weight of the cubes (g)</th>
+    <th>Number of cells per g of cube</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q cubes</td>
+    <td>13,30</td>
+    <td>6,6x10<sup>6</sup></td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R cubes</td>
+    <td>13,39</td>
+    <td>5,9x10<sup>6</sup></td>
+  </tr>
+  <tr>
+    <td>G1513 in R cubes</td>
+    <td>13,39</td>
+    <td>3,9x10<sup>8</sup></td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in S cubes</td>
+    <td>10,70</td>
+    <td>4,8x10<sup>8</sup></td>
+  </tr>
+</table>
+We are going to plate one cube of each kind each day and follow the survival rate during a week of drying.
+For the OD, here are the results we obtained (we obtained them on the 10th of August).
+<table style="width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>OD of the sample</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution of the sample</th>
+    <th>Number of colonies in the 10<sup>-4</sup> dilution of the sample</th>
+    <th>Number of colonies in the 10<sup>-5</sup> dilution of the sample</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry</td>
+    <td>1,156</td>
+    <td>+++</td>
+    <td>347</td>
+    <td>23</td>
+  </tr>
+  <tr>
+    <td>G1513</td>
+    <td>0,392</td>
+    <td>+++</td>
+    <td>439</td>
+    <td>/</td>
+  </tr>
+</table>
+So, for Sc. mcherry:<b> 1A=2,5x10<sup>7</sup> cells/ml </b> <br>
+For G1513:<b> 1A=1,1x10<sup>8</sup> cells/ml </b>
+<br>
 <h1 class="date three">September 9th</h1>
+We plated the first cubes today.<br>
+Everyday, we will follow the same protocol:
+<ul>
+<li>Weigh the cube</li>
+<li>Dilute the cube in 10ml of osmosed water during 5min</li>
+<li>Dilute the solution obtained 10<sup>-1</sup>, 10<sup>-2</sup> and 10<sup>-3</sup> times</li>
+<li>Plate the dilutions</li>
+</ul>
+This should only take 20min in total, doing it for all the cubes in the same time.<br>
+For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating).
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-4</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>0,96</td>
+    <td>6,3x10<sup>6</sup></td>
+    <td>72</td>
+    <td>10</td>
+    <td>8,6x10<sup>6</sup></td>
+    <td>136,5%</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>0,96</td>
+    <td>5,7x10<sup>6</sup></td>
+    <td>80</td>
+    <td>11</td>
+    <td>9,5x10<sup>6</sup></td>
+    <td>166,7%</td>
+  </tr>
-<br><br>
+  <tr>
+    <td>G1513 in R</td>
+    <td>0,96</td>
+    <td>3,7x10<sup>8</sup></td>
+    <td>79</td>
+    <td>10</td>
+    <td>9,0x10<sup>6</sup></td>
+    <td>2,4%</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>0,72</td>
+    <td>5,4x10<sup>8</sup></td>
+    <td>357</td>
+    <td>23</td>
+    <td>2,9x10<sup>7</sup></td>
+    <td>5,4%</td>
+  </tr>
+</table>
+<br>
 <h1 class="date three">September 10th</h1>
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-4</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>0,97</td>
+    <td>6,4x10<sup>6</sup></td>
+    <td>104</td>
+    <td>5</td>
+    <td>7,7x10<sup>6</sup></td>
+    <td>120,3</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>1,40</td>
+    <td>8,3x10<sup>6</sup></td>
+    <td>150</td>
+    <td>11</td>
+    <td>1,3x10<sup>7</sup></td>
+    <td>156,6</td>
+  </tr>
+  <tr>
+    <td>G1513 in R</td>
+    <td>1,40</td>
+    <td>5,5x10<sup>8</sup></td>
+    <td>126</td>
+    <td>/</td>
+    <td>1,3x10<sup>7</sup></td>
+    <td>2,4</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>1,19</td>
+    <td>5,7x10<sup>8</sup></td>
+    <td>63</td>
+    <td>/</td>
+    <td>6,3x10<sup>6</sup></td>
+    <td>1,1%</td>
+  </tr>
+</table>
+<br>
+<h1 class="date three">September 11th</h1>
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-2</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>1,04</td>
+    <td>6,9x10<sup>6</sup></td>
+    <td></td>
+    <td>77</td>
+    <td>7,7x10<sup>6</sup></td>
+    <td>111,5%</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>1,28</td>
+    <td>8,5x10<sup>6</sup></td>
+    <td></td>
+    <td>149</td>
+    <td>1,5x10<sup>7</sup></td>
+    <td>176,5</td>
+  </tr>
+  <tr>
+    <td>G1513 in R</td>
+    <td>1,28</td>
+    <td>5,0x10<sup>8</sup></td>
+    <td>164</td>
+    <td>/</td>
+    <td>1,6x10<sup>6</sup></td>
+    <td>0,3%</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>1,49</td>
+    <td>5,8x10<sup>8</sup></td>
+    <td>299</td>
+    <td>/</td>
+    <td>3,0x10<sup>6</sup></td>
+    <td>0,5%</td>
+  </tr>
+</table>
+<br>
+<h1 class="date three">September 12th</h1>
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-2</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>1,50</td>
+    <td>9,9x10<sup>6</sup></td>
+    <td>877</td>
+    <td>88</td>
+    <td>1,3x10<sup>7</sup></td>
+    <td>131,3%</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>1,12</td>
+    <td>6,6x10<sup>6</sup></td>
+    <td>513</td>
+    <td>52</td>
+    <td>7,2x10<sup>6</sup></td>
+    <td>109,1%</td>
+  </tr>
+  <tr>
+    <td>G1513 in R</td>
+    <td>1,12</td>
+    <td>4,4x10<sup>8</sup></td>
+    <td>331</td>
+    <td>/</td>
+    <td>3,3x10<sup>6</sup></td>
+    <td>0,8%</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>0,93</td>
+    <td>4,5x10<sup>8</sup></td>
+    <td>368</td>
+    <td>/</td>
+    <td>3,7x10<sup>6</sup></td>
+    <td>0,8%</td>
+  </tr>
+</table>
+<br>
+<h1 class="date three">September 13th</h1>
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-2</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>1,17</td>
+    <td>7,7x10<sup>6</sup></td>
+    <td>/</td>
+    <td>123</td>
+    <td>1,2x10<sup>7</sup></td>
+    <td>155,8%</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>1,50</td>
+    <td>8,9x10<sup>6</sup></td>
+    <td>/</td>
+    <td>117</td>
+    <td>1,2x10<sup>7</sup></td>
+    <td>134,8%</td>
+  </tr>
+  <tr>
+    <td>G1513 in R</td>
+    <td>1,50</td>
+    <td>5,9x10<sup>8</sup></td>
+    <td>28</td>
+    <td>/</td>
+    <td>2,8x10<sup>5</sup></td>
+    <td>0,05%</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>0,96</td>
+    <td>4,6x10<sup>8</sup></td>
+    <td>30</td>
+    <td>/</td>
+    <td>3,0x10<sup>5</sup></td>
+    <td>0,07%</td>
+  </tr>
+</table>
+<br>
+<h1 class="date three">September 14th</h1>
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-2</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>1,18</td>
+    <td>7,8x10<sup>6</sup></td>
+    <td>/</td>
+    <td>103</td>
+    <td>1,0x10<sup>7</sup></td>
+    <td>128,2%</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>1,33</td>
+    <td>7,9x10<sup>6</sup></td>
+    <td>/</td>
+    <td>83</td>
+    <td>8,3x10<sup>6</sup></td>
+    <td>105,1%</td>
+  </tr>
+  <tr>
+    <td>G1513 in R</td>
+    <td>1,33</td>
+    <td>5,2x10<sup>8</sup></td>
+    <td>12</td>
+    <td>/</td>
+    <td>1,2x10<sup>5</sup></td>
+    <td>0,02%</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>0,82</td>
+    <td>3,9x10<sup>8</sup></td>
+    <td>49</td>
+    <td>/</td>
+    <td>4,9x10<sup>5</sup></td>
+    <td>0,1%</td>
+  </tr>
+</table>
+<br>
+<h1 class="date three">September 15th</h1>
+<table style="font-size: 0.7em; width:100%; text-align:center">
+  <tr>
+    <th>Studied strains</th>
+    <th>Weight (g)</th>
+    <th>Number of cells initially in the cube</th>
+    <th>Number of colonies in the 10<sup>-2</sup> dilution</th>
+    <th>Number of colonies in the 10<sup>-3</sup> dilution</th>
+    <th>Number of cells in the cube after the drying period</th>
+    <th>Survival rate</th>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in Q</td>
+    <td>1,08</td>
+    <td>7,1x10<sup>6</sup></td>
+    <td>/</td>
+    <td>91</td>
+    <td>9,1x10<sup>6</sup></td>
+    <td>128,2</td>
+  </tr>
+  <tr>
+    <td>Sc. mcherry in R</td>
+    <td>1,44</td>
+    <td>8,5x10<sup>6</sup></td>
+    <td>/</td>
+    <td>84</td>
+    <td>8,4x10<sup>6</sup></td>
+    <td>98,8</td>
+  </tr>
+  <tr>
+    <td>G1513 in R</td>
+    <td>1,44</td>
+    <td>5,6x10<sup>8</sup></td>
+    <td>4</td>
+    <td>/</td>
+    <td>4,0x10<sup>4</sup></td>
+    <td>0,0%</td>
+  </tr>
+  <tr>
+    <td>G1513 in S</td>
+    <td>1,01</td>
+    <td>4,9x10<sup>8</sup></td>
+    <td>10</td>
+    <td>/</td>
+    <td>1,0x10<sup>5</sup></td>
+    <td>0,0%</td>
+  </tr>
+</table>
 </html>

Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"

Latest revision as of 12:55, 21 October 2015

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

Notebook - Manufacturing

July 27th

Beginning of the manufacturing project! Let's do it!

Goal

Material

Procedure

July 28th

Goal

Material

Procedure

Results and discussion

July 29th

Result of the OD test made on the 27th of July:

July 30th

Results of the drying test made on the 28th of July:

Procedure

July 31th

Result of the OD test made on the 29th of July:

August 1st

Result of the drying test made on the 30th of July:

Discussion and New Goal

Material

Procedure

August 3rd

Continuing the powder recipe we began on the 1st of August

August 4th

Discussion and New Goal

Procedure

Result

August 5th

Results of the survival of Sc. mcherry in the potato powder made on the 3rd of August:

Discussion:

August 6th

Results of the survival of Sc. mcherry in the cubes made on the 4th of August:

August 7th

Goal

Material

Procedure

August 10th

Results of the OD test for G1513 made on the 7th of August:

Procedure

August 11th

Goal

Material

Procedure

August 12th

Results of the cube tests made on the 10th of August

Goal

August 13th

Results of the media test made on the 11th of August

August 17th

Procedure

Results of the media test made on the 13th of August

August 18th

Results of the OD test made on the 17th of August

August 19th

Results of the cube test made on the 17th of August

August 20th

Procedure

August 24th

Procedure

Results of the OD test made on the 20th of August

Results of the cube test made on the 20th of August

August 25th

Results of the OD tests made on the 24th of August

August 26th

Results of the cube test made on the 24th of August

August 27th

Goal

Material

Procedure

August 28th

Goal

Procedure

August 31th

Results of the media test made on the 27th of August