Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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{{Paris_Bettencourt/header}} | {{Paris_Bettencourt/header}} | ||
{{Paris_Bettencourt/menu}} | {{Paris_Bettencourt/menu}} | ||
− | {{Paris_Bettencourt/ | + | {{Paris_Bettencourt/banner|page_id=notebook|page_name=Notebook - Manufacturing}} |
<html> | <html> | ||
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The goal of this project is to create an way to grow and distribute our strains in India, easily and for cheap.<br> | The goal of this project is to create an way to grow and distribute our strains in India, easily and for cheap.<br> | ||
Since our genetically engineered strains will be ready only in september, we will not have time to work on it, so we have to find similar strains, that we can study and select with antibiotics.<br> | Since our genetically engineered strains will be ready only in september, we will not have time to work on it, so we have to find similar strains, that we can study and select with antibiotics.<br> | ||
− | We chose Saccharomyces cerevisiae mcherry, wich is resistant to geneticin at a concentration of 200µg/mL, and produce RFP.<br> | + | We chose Saccharomyces cerevisiae mcherry, wich is resistant to geneticin at a concentration of 200µg/mL, and produce RFP. Therefore, it's easy to select and characterize it.<br> |
Basically, we are going to try different ways to distribute the strains and see how well they survived for each process.<br> | Basically, we are going to try different ways to distribute the strains and see how well they survived for each process.<br> | ||
In order to do that, we will have to calculate a survival rate so we have to be able to know how many cells we are putting in the beggining, in the recipe we are trying. | In order to do that, we will have to calculate a survival rate so we have to be able to know how many cells we are putting in the beggining, in the recipe we are trying. | ||
Line 97: | Line 97: | ||
The 10ml solution of Sc. mcherry/water had an OD of 2,557.<br> | The 10ml solution of Sc. mcherry/water had an OD of 2,557.<br> | ||
100µL of the 10<sup>-4</sup> dilution grew 359 colonies, so:<br> | 100µL of the 10<sup>-4</sup> dilution grew 359 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 1,4x10<sup>7</sup> cells/ml</b><br> |
We repeated the very same OD experiment today, for more repetitions. | We repeated the very same OD experiment today, for more repetitions. | ||
Line 129: | Line 129: | ||
The 10ml solution of Sc. mcherry/water had an OD of 2,635.<br> | The 10ml solution of Sc. mcherry/water had an OD of 2,635.<br> | ||
100µL of the 10<sup>-4</sup> dilution grew 205 colonies, so:<br> | 100µL of the 10<sup>-4</sup> dilution grew 205 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 7,8x10<sup>6</sup> cells/ml</b> |
− | <br>< | + | <br> |
+ | <a name="august" class="anchor"><h1></h1></a> | ||
<h1 class="date two">August 1st</h1> | <h1 class="date two">August 1st</h1> | ||
<h2>Result of the drying test made on the 30th of July:</h2> | <h2>Result of the drying test made on the 30th of July:</h2> | ||
Line 144: | Line 145: | ||
<br> | <br> | ||
Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br> | Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br> | ||
− | We found a recipe that inspired us <a href="www.instructables.com">here</a>. <br> | + | We found a recipe that inspired us <a href="http://www.instructables.com/id/Stop-Paying-for-Yeast-Make-Your-Own/">here</a>. <br> |
We decided to compare the initial recipe to our modifications, so we made for different tests :<br> | We decided to compare the initial recipe to our modifications, so we made for different tests :<br> | ||
<ul> | <ul> | ||
Line 241: | Line 242: | ||
Survival rate: | Survival rate: | ||
<ul> | <ul> | ||
− | <li>Wheat flour, sugar and ginger: | + | <li>Wheat flour, sugar and ginger: 14% </li> |
− | <li>Rice flour, sugar and ginger: | + | <li>Rice flour, sugar and ginger: 20%</li> |
− | <li>Rice flour, sugar: | + | <li>Rice flour, sugar: 12%</li> |
− | <li>Rice flour: | + | <li>Rice flour: 4%</li> |
</ul> | </ul> | ||
<h2>Discussion:</h2> | <h2>Discussion:</h2> | ||
Line 256: | Line 257: | ||
<div class="column-left"><p> | <div class="column-left"><p> | ||
<br> | <br> | ||
− | We obtained a survival rate of | + | We obtained a survival rate of 69%. <br><br> |
After staying 5hours in the water, the survival rate reached 737%. | After staying 5hours in the water, the survival rate reached 737%. | ||
</p></div> | </p></div> | ||
Line 318: | Line 319: | ||
We have, in average, 7,74x10<sup>3</sup> cells per ml of the 10<sup>-1</sup> dilution.<br> | We have, in average, 7,74x10<sup>3</sup> cells per ml of the 10<sup>-1</sup> dilution.<br> | ||
− | so: <b>1 | + | so: <b>1 A = 6,0x10<sup>4</sup> cells/ml</b><br> |
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
Line 398: | Line 399: | ||
The 10ml solution of Sc. mcherry/water had an OD of 1,820.<br> | The 10ml solution of Sc. mcherry/water had an OD of 1,820.<br> | ||
100µL of the 10<sup>-4</sup> dilution grew 70 colonies, so:<br> | 100µL of the 10<sup>-4</sup> dilution grew 70 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 3,8x10<sup>6</sup> cells/ml</b><br> |
For G1513:<br> | For G1513:<br> | ||
Line 417: | Line 418: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | So <b>1 | + | So <b>1 A = 1,1x10<sup>8</sup> cells/ml</b> |
Line 448: | Line 449: | ||
The 10ml solution of Sc. mcherry/water had an OD of 1,828.<br> | The 10ml solution of Sc. mcherry/water had an OD of 1,828.<br> | ||
100µL of the 10<sup>-5</sup> dilution grew 56 colonies, so:<br> | 100µL of the 10<sup>-5</sup> dilution grew 56 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 3,1x10<sup>6</sup> cells/ml</b><br> |
For G1513:<br> | For G1513:<br> | ||
Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | ||
Line 478: | Line 479: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <b>1 | + | <b>1 A = 2,3x10<sup>6</sup> cells/mL</b><br> |
The second 10ml solution of Sc. mcherry/water had an OD of 2,523.<br> | The second 10ml solution of Sc. mcherry/water had an OD of 2,523.<br> | ||
100µL of the 10<sup>-5</sup> dilution grew 95 colonies.<br> | 100µL of the 10<sup>-5</sup> dilution grew 95 colonies.<br> | ||
− | so <b>1 | + | so <b>1 A = 3,8x10<sup>6</sup> cells/mL</b><br> |
For G1513:<br> | For G1513:<br> | ||
Nothing... We used cells from the previous culture, wich was dead, so it seems normal. | Nothing... We used cells from the previous culture, wich was dead, so it seems normal. | ||
Line 523: | Line 524: | ||
<h2>Goal</h2> | <h2>Goal</h2> | ||
We want to see how the strains in the cubes react when put in idli.<br> | We want to see how the strains in the cubes react when put in idli.<br> | ||
− | |||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
<ul> | <ul> | ||
Line 530: | Line 530: | ||
<li>Dilute the cube in 5ml of osmosed water</li> | <li>Dilute the cube in 5ml of osmosed water</li> | ||
<li>Add this solution to 1 bottle of idli before the fermentation</li> | <li>Add this solution to 1 bottle of idli before the fermentation</li> | ||
+ | <li>After the fermentation, plate 100μL of a 10<sup>-2</sup> dilution of the Idli water</li> | ||
</ul> | </ul> | ||
Line 540: | Line 541: | ||
<h2>Results of the idli test</h2> | <h2>Results of the idli test</h2> | ||
− | + | <table style="width:100%; text-align:center"> | |
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Number of cells in the top part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | <th>Number of cells in the middle part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | <th>Number of cells in the bottom part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>X</th> | ||
+ | <td>38 / 23</td> | ||
+ | <td>324</td> | ||
+ | <td>71 / 111</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Y</th> | ||
+ | <td>0</td> | ||
+ | <td>39 / 27</td> | ||
+ | <td>64 / 47</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
− | < | + | <a name="september" class="anchor"><h1 class="date three">September 2nd</h1></a> |
− | <h1 class="date three">September 2nd</h1> | + | |
<h2>Results of the second media test made on the 31st of August</h2> | <h2>Results of the second media test made on the 31st of August</h2> | ||
It's a fail, there was almost only water in the well so nothing grew.<br> | It's a fail, there was almost only water in the well so nothing grew.<br> | ||
Line 569: | Line 589: | ||
<h1 class="date three">September 8th</h1> | <h1 class="date three">September 8th</h1> | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
− | Today we used the fresh cultures we made the 6th to make lots of new cubes and OD test. | + | Today we used the fresh cultures we made on the 6th of August to make lots of new cubes and OD test.<br> |
− | + | After centrifugation, throwing the media and replacing it by osmosed water, the cultures gave us two solutions: | |
+ | <ul><li>Solution X: 8,8x10<sup>7</sup> cells of Sc. mcherry per ml</li> | ||
+ | <li>Solution Y: 5,2x10<sup>9</sup> cells of G1513 per ml</li></ul> | ||
− | < | + | We made 3 different types of cubes: |
− | < | + | <ul> |
+ | <li>Cubes Q: 15ml of rice flour, 6ml of osmosed water and 1ml of solution X</li> | ||
+ | <li>Cubes R: 15ml of rice flour, 5ml of osmosed water, 900μL of solution X and 1ml of solution Y</li> | ||
+ | <li>Cubes S: 15ml of rice flour, 6ml of osmosed water and 1ml of solution Y</li> | ||
+ | </ul> | ||
+ | <table style="width:100%; text-align:center"> | ||
+ | <tr> | ||
+ | <th>Strains and cubes</th> | ||
+ | <th>Total weight of the cubes (g)</th> | ||
+ | <th>Number of cells per g of cube</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sc. mcherry in Q cubes</td> | ||
+ | <td>13,30</td> | ||
+ | <td>6,6x10<sup>6</sup></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sc. mcherry in R cubes</td> | ||
+ | <td>13,39</td> | ||
+ | <td>5,9x10<sup>6</sup></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>G1513 in R cubes</td> | ||
+ | <td>13,39</td> | ||
+ | <td>3,9x10<sup>8</sup></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sc. mcherry in S cubes</td> | ||
+ | <td>10,70</td> | ||
+ | <td>4,8x10<sup>8</sup></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | We are going to plate one cube of each kind each day and follow the survival rate during a week of drying. | ||
+ | For the OD, here are the results we obtained (we obtained them on the 10th of August). | ||
<table style="width:100%; text-align:center"> | <table style="width:100%; text-align:center"> | ||
+ | <tr> | ||
+ | <th>Studied strains</th> | ||
+ | <th>OD of the sample</th> | ||
+ | <th>Number of colonies in the 10<sup>-3</sup> dilution of the sample</th> | ||
+ | <th>Number of colonies in the 10<sup>-4</sup> dilution of the sample</th> | ||
+ | <th>Number of colonies in the 10<sup>-5</sup> dilution of the sample</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sc. mcherry</td> | ||
+ | <td>1,156</td> | ||
+ | <td>+++</td> | ||
+ | <td>347</td> | ||
+ | <td>23</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>G1513</td> | ||
+ | <td>0,392</td> | ||
+ | <td>+++</td> | ||
+ | <td>439</td> | ||
+ | <td>/</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | So, for Sc. mcherry:<b> 1A=2,5x10<sup>7</sup> cells/ml </b> <br> | ||
+ | For G1513:<b> 1A=1,1x10<sup>8</sup> cells/ml </b> | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <h1 class="date three">September 9th</h1> | ||
+ | We plated the first cubes today.<br> | ||
+ | Everyday, we will follow the same protocol: | ||
+ | <ul> | ||
+ | <li>Weigh the cube</li> | ||
+ | <li>Dilute the cube in 10ml of osmosed water during 5min</li> | ||
+ | <li>Dilute the solution obtained 10<sup>-1</sup>, 10<sup>-2</sup> and 10<sup>-3</sup> times</li> | ||
+ | <li>Plate the dilutions</li> | ||
+ | </ul> | ||
+ | This should only take 20min in total, doing it for all the cubes in the same time.<br> | ||
+ | For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating). | ||
+ | <table style="font-size: 0.7em; width:100%; text-align:center"> | ||
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
− | + | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-4</sup> dilution</th> | <th>Number of colonies in the 10<sup>-4</sup> dilution</th> | ||
Line 589: | Line 685: | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
<td>0,96</td> | <td>0,96</td> | ||
− | <td>< | + | <td>6,3x10<sup>6</sup></td> |
− | + | <td>72</td> | |
− | <td></td> | + | |
<td>10</td> | <td>10</td> | ||
− | <td></td> | + | <td>8,6x10<sup>6</sup></td> |
− | <td></td> | + | <td>136,5%</td> |
</tr> | </tr> | ||
Line 600: | Line 695: | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
<td>0,96</td> | <td>0,96</td> | ||
− | <td>< | + | <td>5,7x10<sup>6</sup></td> |
− | + | <td>80</td> | |
− | <td></td> | + | |
<td>11</td> | <td>11</td> | ||
− | <td></td> | + | <td>9,5x10<sup>6</sup></td> |
− | <td></td> | + | <td>166,7%</td> |
</tr> | </tr> | ||
Line 611: | Line 705: | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
<td>0,96</td> | <td>0,96</td> | ||
− | <td>< | + | <td>3,7x10<sup>8</sup></td> |
− | + | <td>79</td> | |
− | <td></td> | + | |
<td>10</td> | <td>10</td> | ||
− | <td></td> | + | <td>9,0x10<sup>6</sup></td> |
− | <td></td> | + | <td>2,4%</td> |
</tr> | </tr> | ||
Line 622: | Line 715: | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
<td>0,72</td> | <td>0,72</td> | ||
− | <td>< | + | <td>5,4x10<sup>8</sup></td> |
− | + | <td>357</td> | |
− | <td></td> | + | |
<td>23</td> | <td>23</td> | ||
− | <td></td> | + | <td>2,9x10<sup>7</sup></td> |
− | <td></td> | + | <td>5,4%</td> |
</tr> | </tr> | ||
− | + | </table> | |
<br> | <br> | ||
<h1 class="date three">September 10th</h1> | <h1 class="date three">September 10th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
− | + | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-4</sup> dilution</th> | <th>Number of colonies in the 10<sup>-4</sup> dilution</th> | ||
Line 647: | Line 738: | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
<td>0,97</td> | <td>0,97</td> | ||
− | <td>< | + | <td>6,4x10<sup>6</sup></td> |
− | + | <td>104</td> | |
− | <td></td> | + | <td>5</td> |
− | <td> | + | <td>7,7x10<sup>6</sup></td> |
− | <td></td> | + | <td>120,3</td> |
− | <td></td> | + | |
</tr> | </tr> | ||
Line 658: | Line 748: | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
<td>1,40</td> | <td>1,40</td> | ||
− | <td>< | + | <td>8,3x10<sup>6</sup></td> |
− | + | <td>150</td> | |
− | <td></td> | + | <td>11</td> |
− | <td> | + | <td>1,3x10<sup>7</sup></td> |
− | <td></td> | + | <td>156,6</td> |
− | <td></td> | + | |
</tr> | </tr> | ||
Line 669: | Line 758: | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
<td>1,40</td> | <td>1,40</td> | ||
− | <td>< | + | <td>5,5x10<sup>8</sup></td> |
− | + | <td>126</td> | |
− | <td> | + | <td>/</td> |
− | <td></td> | + | <td>1,3x10<sup>7</sup></td> |
− | <td></td> | + | <td>2,4</td> |
− | <td></td> | + | |
</tr> | </tr> | ||
Line 680: | Line 768: | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
<td>1,19</td> | <td>1,19</td> | ||
− | <td>< | + | <td>5,7x10<sup>8</sup></td> |
− | + | <td>63</td> | |
− | <td> | + | <td>/</td> |
− | <td></td> | + | <td>6,3x10<sup>6</sup></td> |
− | <td></td> | + | <td>1,1%</td> |
− | <td></td> | + | |
</tr> | </tr> | ||
− | + | </table> | |
<br> | <br> | ||
<h1 class="date three">September 11th</h1> | <h1 class="date three">September 11th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
<th>Number of colonies in the 10<sup>-2</sup> dilution</th> | <th>Number of colonies in the 10<sup>-2</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
Line 704: | Line 791: | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
<td>1,04</td> | <td>1,04</td> | ||
− | <td></td> | + | <td>6,9x10<sup>6</sup></td> |
<td></td> | <td></td> | ||
<td>77</td> | <td>77</td> | ||
− | <td></td> | + | <td>7,7x10<sup>6</sup></td> |
− | <td></td> | + | <td>111,5%</td> |
</tr> | </tr> | ||
Line 714: | Line 801: | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
<td>1,28</td> | <td>1,28</td> | ||
− | <td></td> | + | <td>8,5x10<sup>6</sup></td> |
<td></td> | <td></td> | ||
<td>149</td> | <td>149</td> | ||
− | <td></td> | + | <td>1,5x10<sup>7</sup></td> |
− | <td></td> | + | <td>176,5</td> |
</tr> | </tr> | ||
Line 724: | Line 811: | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
<td>1,28</td> | <td>1,28</td> | ||
− | <td></td> | + | <td>5,0x10<sup>8</sup></td> |
<td>164</td> | <td>164</td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>1,6x10<sup>6</sup></td> |
− | <td></td> | + | <td>0,3%</td> |
</tr> | </tr> | ||
Line 734: | Line 821: | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
<td>1,49</td> | <td>1,49</td> | ||
− | <td></td> | + | <td>5,8x10<sup>8</sup></td> |
<td>299</td> | <td>299</td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>3,0x10<sup>6</sup></td> |
− | <td></td> | + | <td>0,5%</td> |
</tr> | </tr> | ||
− | + | </table> | |
<br> | <br> | ||
<h1 class="date three">September 12th</h1> | <h1 class="date three">September 12th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
<th>Number of colonies in the 10<sup>-2</sup> dilution</th> | <th>Number of colonies in the 10<sup>-2</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
Line 757: | Line 844: | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
<td>1,50</td> | <td>1,50</td> | ||
− | <td></td> | + | <td>9,9x10<sup>6</sup></td> |
<td>877</td> | <td>877</td> | ||
− | <td></td> | + | <td>88</td> |
− | <td></td> | + | <td>1,3x10<sup>7</sup></td> |
− | <td></td> | + | <td>131,3%</td> |
</tr> | </tr> | ||
Line 767: | Line 854: | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
<td>1,12</td> | <td>1,12</td> | ||
− | <td></td> | + | <td>6,6x10<sup>6</sup></td> |
<td>513</td> | <td>513</td> | ||
− | <td></td> | + | <td>52</td> |
− | <td></td> | + | <td>7,2x10<sup>6</sup></td> |
− | <td></td> | + | <td>109,1%</td> |
</tr> | </tr> | ||
Line 777: | Line 864: | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
<td>1,12</td> | <td>1,12</td> | ||
− | <td></td> | + | <td>4,4x10<sup>8</sup></td> |
<td>331</td> | <td>331</td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>3,3x10<sup>6</sup></td> |
− | <td></td> | + | <td>0,8%</td> |
</tr> | </tr> | ||
Line 787: | Line 874: | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
<td>0,93</td> | <td>0,93</td> | ||
− | <td></td> | + | <td>4,5x10<sup>8</sup></td> |
<td>368</td> | <td>368</td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>3,7x10<sup>6</sup></td> |
− | <td></td> | + | <td>0,8%</td> |
</tr> | </tr> | ||
+ | </table> | ||
<br> | <br> | ||
<h1 class="date three">September 13th</h1> | <h1 class="date three">September 13th</h1> | ||
− | + | <table style="font-size: 0.7em; width:100%; text-align:center"> | |
− | <table style="width:100%; text-align:center"> | + | |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
<th>Number of colonies in the 10<sup>-2</sup> dilution</th> | <th>Number of colonies in the 10<sup>-2</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
Line 811: | Line 898: | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
<td>1,17</td> | <td>1,17</td> | ||
− | <td></td> | + | <td>7,7x10<sup>6</sup></td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>123</td> |
− | <td></td> | + | <td>1,2x10<sup>7</sup></td> |
− | <td></td> | + | <td>155,8%</td> |
</tr> | </tr> | ||
Line 821: | Line 908: | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
<td>1,50</td> | <td>1,50</td> | ||
− | <td></td> | + | <td>8,9x10<sup>6</sup></td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>117</td> |
− | <td></td> | + | <td>1,2x10<sup>7</sup></td> |
− | <td></td> | + | <td>134,8%</td> |
</tr> | </tr> | ||
Line 831: | Line 918: | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
<td>1,50</td> | <td>1,50</td> | ||
− | <td></td> | + | <td>5,9x10<sup>8</sup></td> |
<td>28</td> | <td>28</td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>2,8x10<sup>5</sup></td> |
− | <td></td> | + | <td>0,05%</td> |
</tr> | </tr> | ||
Line 841: | Line 928: | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
<td>0,96</td> | <td>0,96</td> | ||
− | <td></td> | + | <td>4,6x10<sup>8</sup></td> |
<td>30</td> | <td>30</td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>3,0x10<sup>5</sup></td> |
− | <td></td> | + | <td>0,07%</td> |
</tr> | </tr> | ||
+ | </table> | ||
<br> | <br> | ||
<h1 class="date three">September 14th</h1> | <h1 class="date three">September 14th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
<th>Number of colonies in the 10<sup>-2</sup> dilution</th> | <th>Number of colonies in the 10<sup>-2</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
Line 863: | Line 951: | ||
<tr> | <tr> | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
− | <td>1, | + | <td>1,18</td> |
− | <td></td> | + | <td>7,8x10<sup>6</sup></td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>103</td> |
− | <td></td> | + | <td>1,0x10<sup>7</sup></td> |
− | <td></td> | + | <td>128,2%</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
− | <td>1, | + | <td>1,33</td> |
− | <td></td> | + | <td>7,9x10<sup>6</sup></td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>83</td> |
− | <td></td> | + | <td>8,3x10<sup>6</sup></td> |
− | <td></td> | + | <td>105,1%</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
− | <td>1, | + | <td>1,33</td> |
− | <td></td> | + | <td>5,2x10<sup>8</sup></td> |
− | <td> | + | <td>12</td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>1,2x10<sup>5</sup></td> |
− | <td></td> | + | <td>0,02%</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
− | <td>0, | + | <td>0,82</td> |
− | <td></td> | + | <td>3,9x10<sup>8</sup></td> |
− | <td> | + | <td>49</td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>4,9x10<sup>5</sup></td> |
− | <td></td> | + | <td>0,1%</td> |
</tr> | </tr> | ||
+ | </table> | ||
<br> | <br> | ||
<h1 class="date three">September 15th</h1> | <h1 class="date three">September 15th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
<th>Weight (g)</th> | <th>Weight (g)</th> | ||
− | <th>Number of cells | + | <th>Number of cells initially in the cube</th> |
<th>Number of colonies in the 10<sup>-2</sup> dilution</th> | <th>Number of colonies in the 10<sup>-2</sup> dilution</th> | ||
<th>Number of colonies in the 10<sup>-3</sup> dilution</th> | <th>Number of colonies in the 10<sup>-3</sup> dilution</th> | ||
Line 915: | Line 1,004: | ||
<tr> | <tr> | ||
<td>Sc. mcherry in Q</td> | <td>Sc. mcherry in Q</td> | ||
− | <td>1, | + | <td>1,08</td> |
− | <td></td> | + | <td>7,1x10<sup>6</sup></td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>91</td> |
− | <td></td> | + | <td>9,1x10<sup>6</sup></td> |
− | <td></td> | + | <td>128,2</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Sc. mcherry in R</td> | <td>Sc. mcherry in R</td> | ||
− | <td>1, | + | <td>1,44</td> |
− | <td></td> | + | <td>8,5x10<sup>6</sup></td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>84</td> |
− | <td></td> | + | <td>8,4x10<sup>6</sup></td> |
− | <td></td> | + | <td>98,8</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>G1513 in R</td> | <td>G1513 in R</td> | ||
− | <td>1, | + | <td>1,44</td> |
− | <td></td> | + | <td>5,6x10<sup>8</sup></td> |
− | <td> | + | <td>4</td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>4,0x10<sup>4</sup></td> |
− | <td></td> | + | <td>0,0%</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>G1513 in S</td> | <td>G1513 in S</td> | ||
− | <td> | + | <td>1,01</td> |
− | <td></td> | + | <td>4,9x10<sup>8</sup></td> |
− | <td> | + | <td>10</td> |
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>1,0x10<sup>5</sup></td> |
− | <td></td> | + | <td>0,0%</td> |
</tr> | </tr> | ||
+ | </table> | ||
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</html> | </html> |
Latest revision as of 12:55, 21 October 2015