Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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{{Paris_Bettencourt/header}} | {{Paris_Bettencourt/header}} | ||
{{Paris_Bettencourt/menu}} | {{Paris_Bettencourt/menu}} | ||
− | {{Paris_Bettencourt/ | + | {{Paris_Bettencourt/banner|page_id=notebook|page_name=Notebook - Manufacturing}} |
<html> | <html> | ||
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The 10ml solution of Sc. mcherry/water had an OD of 2,557.<br> | The 10ml solution of Sc. mcherry/water had an OD of 2,557.<br> | ||
100µL of the 10<sup>-4</sup> dilution grew 359 colonies, so:<br> | 100µL of the 10<sup>-4</sup> dilution grew 359 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 1,4x10<sup>7</sup> cells/ml</b><br> |
We repeated the very same OD experiment today, for more repetitions. | We repeated the very same OD experiment today, for more repetitions. | ||
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The 10ml solution of Sc. mcherry/water had an OD of 2,635.<br> | The 10ml solution of Sc. mcherry/water had an OD of 2,635.<br> | ||
100µL of the 10<sup>-4</sup> dilution grew 205 colonies, so:<br> | 100µL of the 10<sup>-4</sup> dilution grew 205 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 7,8x10<sup>6</sup> cells/ml</b> |
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<br> | <br> | ||
Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br> | Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br> | ||
− | We found a recipe that inspired us <a href="www.instructables.com">here</a>. <br> | + | We found a recipe that inspired us <a href="http://www.instructables.com/id/Stop-Paying-for-Yeast-Make-Your-Own/">here</a>. <br> |
We decided to compare the initial recipe to our modifications, so we made for different tests :<br> | We decided to compare the initial recipe to our modifications, so we made for different tests :<br> | ||
<ul> | <ul> | ||
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<div class="column-left"><p> | <div class="column-left"><p> | ||
<br> | <br> | ||
− | We obtained a survival rate of | + | We obtained a survival rate of 69%. <br><br> |
After staying 5hours in the water, the survival rate reached 737%. | After staying 5hours in the water, the survival rate reached 737%. | ||
</p></div> | </p></div> | ||
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We have, in average, 7,74x10<sup>3</sup> cells per ml of the 10<sup>-1</sup> dilution.<br> | We have, in average, 7,74x10<sup>3</sup> cells per ml of the 10<sup>-1</sup> dilution.<br> | ||
− | so: <b>1 | + | so: <b>1 A = 6,0x10<sup>4</sup> cells/ml</b><br> |
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
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The 10ml solution of Sc. mcherry/water had an OD of 1,820.<br> | The 10ml solution of Sc. mcherry/water had an OD of 1,820.<br> | ||
100µL of the 10<sup>-4</sup> dilution grew 70 colonies, so:<br> | 100µL of the 10<sup>-4</sup> dilution grew 70 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 3,8x10<sup>6</sup> cells/ml</b><br> |
For G1513:<br> | For G1513:<br> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
− | So <b>1 | + | So <b>1 A = 1,1x10<sup>8</sup> cells/ml</b> |
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The 10ml solution of Sc. mcherry/water had an OD of 1,828.<br> | The 10ml solution of Sc. mcherry/water had an OD of 1,828.<br> | ||
100µL of the 10<sup>-5</sup> dilution grew 56 colonies, so:<br> | 100µL of the 10<sup>-5</sup> dilution grew 56 colonies, so:<br> | ||
− | <b>1 | + | <b>1 A = 3,1x10<sup>6</sup> cells/ml</b><br> |
For G1513:<br> | For G1513:<br> | ||
Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
− | <b>1 | + | <b>1 A = 2,3x10<sup>6</sup> cells/mL</b><br> |
The second 10ml solution of Sc. mcherry/water had an OD of 2,523.<br> | The second 10ml solution of Sc. mcherry/water had an OD of 2,523.<br> | ||
100µL of the 10<sup>-5</sup> dilution grew 95 colonies.<br> | 100µL of the 10<sup>-5</sup> dilution grew 95 colonies.<br> | ||
− | so <b>1 | + | so <b>1 A = 3,8x10<sup>6</sup> cells/mL</b><br> |
For G1513:<br> | For G1513:<br> | ||
Nothing... We used cells from the previous culture, wich was dead, so it seems normal. | Nothing... We used cells from the previous culture, wich was dead, so it seems normal. | ||
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<h2>Goal</h2> | <h2>Goal</h2> | ||
We want to see how the strains in the cubes react when put in idli.<br> | We want to see how the strains in the cubes react when put in idli.<br> | ||
− | |||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
<ul> | <ul> | ||
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<li>Dilute the cube in 5ml of osmosed water</li> | <li>Dilute the cube in 5ml of osmosed water</li> | ||
<li>Add this solution to 1 bottle of idli before the fermentation</li> | <li>Add this solution to 1 bottle of idli before the fermentation</li> | ||
+ | <li>After the fermentation, plate 100μL of a 10<sup>-2</sup> dilution of the Idli water</li> | ||
</ul> | </ul> | ||
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<h2>Results of the idli test</h2> | <h2>Results of the idli test</h2> | ||
− | + | <table style="width:100%; text-align:center"> | |
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Number of cells in the top part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | <th>Number of cells in the middle part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | <th>Number of cells in the bottom part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>X</th> | ||
+ | <td>38 / 23</td> | ||
+ | <td>324</td> | ||
+ | <td>71 / 111</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Y</th> | ||
+ | <td>0</td> | ||
+ | <td>39 / 27</td> | ||
+ | <td>64 / 47</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
− | <a name="september" class="anchor"> | + | <a name="september" class="anchor"><h1 class="date three">September 2nd</h1></a> |
− | + | ||
− | <h1 class="date three">September 2nd</h1> | + | |
<h2>Results of the second media test made on the 31st of August</h2> | <h2>Results of the second media test made on the 31st of August</h2> | ||
It's a fail, there was almost only water in the well so nothing grew.<br> | It's a fail, there was almost only water in the well so nothing grew.<br> | ||
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After centrifugation, throwing the media and replacing it by osmosed water, the cultures gave us two solutions: | After centrifugation, throwing the media and replacing it by osmosed water, the cultures gave us two solutions: | ||
<ul><li>Solution X: 8,8x10<sup>7</sup> cells of Sc. mcherry per ml</li> | <ul><li>Solution X: 8,8x10<sup>7</sup> cells of Sc. mcherry per ml</li> | ||
− | <li>Solution Y: 5,2x10<sup>9</sup> cells of G1513 per ml</li> | + | <li>Solution Y: 5,2x10<sup>9</sup> cells of G1513 per ml</li></ul> |
We made 3 different types of cubes: | We made 3 different types of cubes: | ||
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<h1 class="date three">September 9th</h1> | <h1 class="date three">September 9th</h1> | ||
We plated the first cubes today.<br> | We plated the first cubes today.<br> | ||
− | Everyday, we will follow the same protocol | + | Everyday, we will follow the same protocol: |
+ | <ul> | ||
+ | <li>Weigh the cube</li> | ||
+ | <li>Dilute the cube in 10ml of osmosed water during 5min</li> | ||
+ | <li>Dilute the solution obtained 10<sup>-1</sup>, 10<sup>-2</sup> and 10<sup>-3</sup> times</li> | ||
+ | <li>Plate the dilutions</li> | ||
+ | </ul> | ||
+ | This should only take 20min in total, doing it for all the cubes in the same time.<br> | ||
For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating). | For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating). | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
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<br> | <br> | ||
<h1 class="date three">September 10th</h1> | <h1 class="date three">September 10th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
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<td>0,97</td> | <td>0,97</td> | ||
<td>6,4x10<sup>6</sup></td> | <td>6,4x10<sup>6</sup></td> | ||
− | <td> | + | <td>104</td> |
− | <td> | + | <td>5</td> |
− | <td> | + | <td>7,7x10<sup>6</sup></td> |
− | <td> | + | <td>120,3</td> |
</tr> | </tr> | ||
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<td>1,40</td> | <td>1,40</td> | ||
<td>8,3x10<sup>6</sup></td> | <td>8,3x10<sup>6</sup></td> | ||
− | <td> | + | <td>150</td> |
− | <td> | + | <td>11</td> |
− | <td>1, | + | <td>1,3x10<sup>7</sup></td> |
− | <td> | + | <td>156,6</td> |
</tr> | </tr> | ||
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<br> | <br> | ||
<h1 class="date three">September 11th</h1> | <h1 class="date three">September 11th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
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<br> | <br> | ||
<h1 class="date three">September 12th</h1> | <h1 class="date three">September 12th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
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<br> | <br> | ||
<h1 class="date three">September 13th</h1> | <h1 class="date three">September 13th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
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<h1 class="date three">September 14th</h1> | <h1 class="date three">September 14th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
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<br> | <br> | ||
<h1 class="date three">September 15th</h1> | <h1 class="date three">September 15th</h1> | ||
− | <table style="width:100%; text-align:center"> | + | <table style="font-size: 0.7em; width:100%; text-align:center"> |
<tr> | <tr> | ||
<th>Studied strains</th> | <th>Studied strains</th> | ||
Line 981: | Line 1,006: | ||
<td>1,08</td> | <td>1,08</td> | ||
<td>7,1x10<sup>6</sup></td> | <td>7,1x10<sup>6</sup></td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>91</td> |
− | <td></td> | + | <td>9,1x10<sup>6</sup></td> |
− | <td></td> | + | <td>128,2</td> |
</tr> | </tr> | ||
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<td>1,44</td> | <td>1,44</td> | ||
<td>8,5x10<sup>6</sup></td> | <td>8,5x10<sup>6</sup></td> | ||
− | <td></td> | + | <td>/</td> |
− | <td></td> | + | <td>84</td> |
− | <td></td> | + | <td>8,4x10<sup>6</sup></td> |
− | <td></td> | + | <td>98,8</td> |
</tr> | </tr> | ||
Line 1,017: | Line 1,042: | ||
</tr> | </tr> | ||
</table> | </table> | ||
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</html> | </html> |
Latest revision as of 12:55, 21 October 2015