Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br> | Our second idea was to cook a homemade powder with edible ingredients easily found in India.<br> | ||
− | We found a recipe that inspired us <a href="www.instructables.com">here</a>. <br> | + | We found a recipe that inspired us <a href="http://www.instructables.com/id/Stop-Paying-for-Yeast-Make-Your-Own/">here</a>. <br> |
We decided to compare the initial recipe to our modifications, so we made for different tests :<br> | We decided to compare the initial recipe to our modifications, so we made for different tests :<br> | ||
<ul> | <ul> | ||
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<h2>Goal</h2> | <h2>Goal</h2> | ||
We want to see how the strains in the cubes react when put in idli.<br> | We want to see how the strains in the cubes react when put in idli.<br> | ||
− | |||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
<ul> | <ul> | ||
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<li>Dilute the cube in 5ml of osmosed water</li> | <li>Dilute the cube in 5ml of osmosed water</li> | ||
<li>Add this solution to 1 bottle of idli before the fermentation</li> | <li>Add this solution to 1 bottle of idli before the fermentation</li> | ||
+ | <li>After the fermentation, plate 100μL of a 10<sup>-2</sup> dilution of the Idli water</li> | ||
</ul> | </ul> | ||
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<h2>Results of the idli test</h2> | <h2>Results of the idli test</h2> | ||
− | + | <table style="width:100%; text-align:center"> | |
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Number of cells in the top part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | <th>Number of cells in the middle part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | <th>Number of cells in the bottom part of the idli, 10<sup>-2</sup> dilution</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>X</th> | ||
+ | <td>38 / 23</td> | ||
+ | <td>324</td> | ||
+ | <td>71 / 111</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Y</th> | ||
+ | <td>0</td> | ||
+ | <td>39 / 27</td> | ||
+ | <td>64 / 47</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
− | <a name="september" class="anchor"> | + | <a name="september" class="anchor"><h1 class="date three">September 2nd</h1></a> |
− | + | ||
− | <h1 class="date three">September 2nd</h1> | + | |
<h2>Results of the second media test made on the 31st of August</h2> | <h2>Results of the second media test made on the 31st of August</h2> | ||
It's a fail, there was almost only water in the well so nothing grew.<br> | It's a fail, there was almost only water in the well so nothing grew.<br> | ||
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<h1 class="date three">September 9th</h1> | <h1 class="date three">September 9th</h1> | ||
We plated the first cubes today.<br> | We plated the first cubes today.<br> | ||
− | Everyday, we will follow the same protocol | + | Everyday, we will follow the same protocol: |
+ | <ul> | ||
+ | <li>Weigh the cube</li> | ||
+ | <li>Dilute the cube in 10ml of osmosed water during 5min</li> | ||
+ | <li>Dilute the solution obtained 10<sup>-1</sup>, 10<sup>-2</sup> and 10<sup>-3</sup> times</li> | ||
+ | <li>Plate the dilutions</li> | ||
+ | </ul> | ||
+ | This should only take 20min in total, doing it for all the cubes in the same time.<br> | ||
For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating). | For more clarity, we will write the results for each cubes on the day we plated it (even if the colonies took 1 or 2 days to grow depending on the strains, so we only add the results 2 days after the plating). | ||
<table style="font-size: 0.7em; width:100%; text-align:center"> | <table style="font-size: 0.7em; width:100%; text-align:center"> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
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</html> | </html> |
Latest revision as of 12:55, 21 October 2015