Difference between revisions of "Team:Paris Bettencourt/Project/VitaminB12"
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− | <td class="noTop">Vitamin B12 is already widely naturally synthesized by some natural producers such as <i> | + | <td class="noTop">Vitamin B12 is already widely naturally synthesized by some natural producers such as <i>Propionibacterium freudenrichii</i> which is GRAS (Generally Recognized As Safe) organism. It is however quite impossible to quickly and cheaply quantify it. |
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<td class="noTop">Introducing <i>Propionibacterium</i> in our idli batter would allow us to highly increase its quantity of vitamin B12. Quantifying vitamin B12 in fermented food in a cheap way was aimed by designing a biosensor based on a vitamin B12 riboswitch associated with a fluorescent protein. | <td class="noTop">Introducing <i>Propionibacterium</i> in our idli batter would allow us to highly increase its quantity of vitamin B12. Quantifying vitamin B12 in fermented food in a cheap way was aimed by designing a biosensor based on a vitamin B12 riboswitch associated with a fluorescent protein. | ||
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<i>Propionibacterium freudenreichii</i> is a bacterium that has the Generally Recognized As Safe (GRAS) status, and is commonly found is cheese such as Emmental. It is a natural big producer of vitamin B12, conferring high amounts of this vitamin on cheese. | <i>Propionibacterium freudenreichii</i> is a bacterium that has the Generally Recognized As Safe (GRAS) status, and is commonly found is cheese such as Emmental. It is a natural big producer of vitamin B12, conferring high amounts of this vitamin on cheese. | ||
− | + | Since the biosynthesis of vitamin B12 is a complex process, we decided to engineer the microbiome of idli by directly adding this bacterium to the batter, in the hope of increasing its vitamin B12 content.</p> | |
<p>As a matter of fact, <i>P. freudenreichii</i> was found to grow well in idli (see figure on the right).</p> | <p>As a matter of fact, <i>P. freudenreichii</i> was found to grow well in idli (see figure on the right).</p> | ||
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− | <h1 class="date two">Cobalamin Biosensor</h1> | + | <h1 class="date two" id="biosensor">Cobalamin Biosensor</h1> |
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<h2>Background</h2> | <h2>Background</h2> |
Latest revision as of 15:36, 12 December 2015