Difference between revisions of "Team:Paris Bettencourt/Project/VitaminA"

Line 72: Line 72:
 
         <tracklabel text='HO-E/YB/I-Poly-KanMX4-HO' labelstyle="font-size:13px"></tracklabel>
 
         <tracklabel text='HO-E/YB/I-Poly-KanMX4-HO' labelstyle="font-size:13px"></tracklabel>
 
       <tracklabel labelstyle="font-size:12px;font-weight:400;fill:#999" text="12,010 bp" vadjust="18"></tracklabel>
 
       <tracklabel labelstyle="font-size:12px;font-weight:400;fill:#999" text="12,010 bp" vadjust="18"></tracklabel>
−
               <trackmarker start='296' end='1075' markerstyle='stroke:#000;fill:#orange;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
+
               <trackmarker start='296' end='1075' markerstyle='stroke:#000;fill:#FF0000;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
 
   <markerlabel text='KanMX4' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
   <markerlabel text='KanMX4' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
−
   <trackmarker start='2897' end='3757' markerstyle='stroke:#000;fill:#f00;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
+
   <trackmarker start='2897' end='3757' markerstyle='stroke:#000;fill:#FF8000;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
 
   <markerlabel text='AmpR' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
   <markerlabel text='AmpR' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
−
   <trackmarker start='3912' end='4531' markerstyle='stroke:#000;fill:#f00;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
+
   <trackmarker start='3912' end='4531' markerstyle='stroke:#000;fill:#FACC2E;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
 
   <markerlabel text='pBR322' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
   <markerlabel text='pBR322' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
Line 84: Line 84:
 
             <markerlabel text='ADH1 promoter' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
             <markerlabel text='ADH1 promoter' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
−
         <trackmarker start='6690' end='7817' markerstyle='stroke:#000;fill:#f00;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
+
         <trackmarker start='6690' end='7817' markerstyle='stroke:#000;fill:#01DF01;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
 
             <markerlabel text="crtE" vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
             <markerlabel text="crtE" vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
Line 90: Line 90:
 
             <markerlabel text='2a' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
             <markerlabel text='2a' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
−
         <trackmarker start='7878' end='9893' markerstyle='stroke:#000;fill:#f00;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
+
         <trackmarker start='7878' end='9893' markerstyle='stroke:#000;fill:#00BFFF;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
 
             <markerlabel text='crtYB' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
             <markerlabel text='crtYB' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
Line 96: Line 96:
 
             <markerlabel text='2a' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
             <markerlabel text='2a' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>
−
         <trackmarker start='9954' end='11696' markerstyle='stroke:#000;fill:#f00;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
+
         <trackmarker start='9954' end='11696' markerstyle='stroke:#000;fill:#8000FF;filter:url(#dropshadow)' arrowendlength="7" arrowendwidth="5" wadjust="10" vadjust="-5">
 
             <markerlabel text='crtI' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
             <markerlabel text='crtI' vadjust="40" hadjust="2" valign="outer" style="font-size:14px;font-weight:400;fill:#000000" showline="1" linevadjust="-15" linevadjust="-15" linelabelstyle="stroke:#cc0;stroke-dasharray:2,2;stroke-width:2px;"></markerlabel>
 
</trackmarker>
 
</trackmarker>

Revision as of 15:53, 13 August 2015

Vitamin A




Bibliography

  • Li, Q., Sun, Z., Li, J. & Zhang, Y. Enhancing beta-carotene production in Saccharomyces cerevisiae by metabolic engineering. FEMS Microbiology Letters 345, 94-101 (2013).
  • Beekwilder, J. et al. Polycistronic expression of a ß-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to ß-ionone production. Journal of Biotechnology (2014).
  • Voth, W.P., Richards, J.D., Shaw, J.M. & Stillman, D.J. Yeast vectors for integration at the HO locus. Nucleic acids research 29, E59-E59 (2001).
  • Gietz, R.D. & Schiestl, R.H. High-efficiency yeast transformation using the LiAc / SS carrier DNA / PEG method. Nature Protocols 2, 31-35 (2008).