Difference between revisions of "Team:Paris Bettencourt/jb"

 
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Latest revision as of 15:31, 14 August 2015


o15.053

HO-Poly-KanMX4-HO amplification (reverse) + tail

TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC

o15.056

gBlock2 amplification (forward) + tail

TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC

o15.057

gBlock2 amplification (reverse) + tail

GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT

o15.058

gBlock3 amplification (forward)

AACGTTATTGTTCGGTCCTCCATTGC

o15.059

gBlock3 amplification (reverse) + tail

GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG

o15.060

gBlock4 amplification (forward)

CTTGAGGCTACAATTCATTGGTCAAATAC

o15.061

gBlock4 amplification (reverse) + tail

TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC

o15.117

forward primer for gblock 1 part 1

CGTCCAAGACATACTGCGTTTACG

o15.118

reverse primer for gblock 1 part 1

GTGTGCAACATTCCCACTACATTTTGAATG

o15.119

forward primer for gblock 1 part 2

CATTCAAAATGTAGTGGGAATGTTGCAC

o15.120

reverse primer for gblock 1 part 2

TGGATTGTTGGGAAGGAAAACTTTCCTT

o15.121

forward primer gblock 1 + restriction site ecor1

TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC

o15.122

reverse primer gblock 1 + tail

AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT

o15.123

forward primer TDH3 from registry with HO binding tail

GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG

o15.124

reverse primer TDH3 from registry with RE binding site

TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC

o15.125

forward primer full gblock 1 with new promoter

GCCCAGAATACCCTCCTTGACAG

o15.126

reverse primer full gblock 1 with new promoter

CATGCTAACCCTTCCTCCAGGCT

o15.127

forward primer gblock 2 + tail

TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC

o15.128

reverse primer gblock 2 + tail

GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT

o15.129

forward primer gblock 3

AACGTTATTGTTCGGTCCTCCATTGC

o15.130

reverse primer gblock 3 + tail

GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG

o15.131

forward primer gblock 4

CTTGAGGCTACAATTCATTGGTCAAATAC

o15.132

reverse primer gblock 4 HO tail

TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC

o15.133

reverse primer gblock 4 HMG tail

CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC

o15.134

forward primer vector HO with HMG

GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG

o15.135

forward primer vector HO without HMG (binds to gBlock4)

TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG

o15.136

reverse primer vector HO (binds to TDH3?)

TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC

o15.137

formard primer HMG+tail with RE

TATATCTAGACCCGCCGCCACCATGCAAAG

o15.138

reverse primer HMG+tail with restriction site

TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG

o15.139

forward primer promoter HMG terminator

TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG

o15.140

reverse primer HMG + tail HO

TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG

o15.141

reverse primer gblock 1 part 1 (binds to part2))

TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG

o15.142

forward primer gblock 1 + tail that binds to HO pl

GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG

o15.143

reverse primer HO + tail crappy ADH1

TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC

o15.144

forward primer gblock 1 + tail TDH3

GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC

o15.145

reverse primer TDH3 + tail for kozac

TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC