Difference between revisions of "Team:Paris Bettencourt/Project/Phytase"

Line 17: Line 17:
  
 
<div class="column-right">
 
<div class="column-right">
−
blablala iaetaiue arsetaiuserti adetaiusretaiudrnetaiue airetaiudrnet
 
 
<img src="https://static.igem.org/mediawiki/2015/b/bb/ParisBettencourt_phyticacid.jpg" width=300px">
 
<img src="https://static.igem.org/mediawiki/2015/b/bb/ParisBettencourt_phyticacid.jpg" width=300px">
 +
<p class="legend"><b>Figure 1:</b> Phytic acid can bind to calcium, magnesium, zinc and iron.</p>
 
</div>
 
</div>
 
<div style="clear:both"></div>
 
<div style="clear:both"></div>

Revision as of 17:58, 14 August 2015

Background

Aims

Results

Cobalamin (vitamin B12) deficiency is widely spread in India, due to diet that is mostly vegetarian. We aimed at introducing a high-level cobalamin producer to the rice batter. Tadaa.

Introduction

Phytic acid (C6H18O24P6) is a molecul wich inhibit the absorption of differents cations like iron, zinc, copper, magnesium, calcium and cobalt. It make insoluble salts around this elements, say phytate. This complexes are not soluble, so it is harmful to digestion and absorption of the minerals wich are essential to growth. They are difficult to find in our alimentation, and the absence of this minerals may be the cause of the food deficiency.

The cereals are the food inside we find the most of phytic acid.

The idli is constituate of cereals principally. The majority of minerals present are not absorb. We want to find a solution to the food deficiency, like the study of vitamines.

Figure 1: Phytic acid can bind to calcium, magnesium, zinc and iron.


Phytase

The solution we find is the phytase. It’s a enzyme wich hydrolysis phytates created by the acid phytic when it link to a mineral. In this way, cations will be liberate and may be absorb by the organism.
So, we have to find a way to this system be integrated in the food, because if we just add the enzyme to the bacterial mirobiome, it will be present only on the beginning and the phytase will be not product.

The phytase it’s a enzyme product naturally by Saccharomyces cerevisiae [Jenny Veide, Thomas Andlid 2006]. The yeast contain negative regulator genes, and because of it, the phytase is product in very small quantity.

The negatives regulators are producted by two important genes in Saccharomyces cerevsiae : PHO80 on chromosome 15 (325.249pb - 326.130pb) and PHO85 on chomosome 16 (492.018pb - 493.037pb). With the deletion of this genes, the phytase may be overproduct.

Design

To test our experiment, we use a kit wich permit to dose the phytic acid by colorimetry. We can do this dosage with HPLC chromatography, but it’s a complicated procedure.

Results


Bibliography

Veide, J. & Andlid, T. Improved extracellular phytase activity in Saccharomyces cerevisiae by modifications in the PHO system. International Journal of Food Microbiology 108, 60-67 (2006).