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| − | <li><a href="#june">June</a></li>
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| | <li><a href="#july">July</a></li> | | <li><a href="#july">July</a></li> |
| | <li><a href="#august">August</a></li> | | <li><a href="#august">August</a></li> |
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| − | <a name="june" class="anchor"><h1>June</h1></a>
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| − | <h2 class="date">June 3rd</h2>
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| − | Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?<br><br>
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| − | <h2 class="date">June 23rd</h2>
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| − | <b>Make <i>Lactobacillus plantarum</i> produce B12</b><br>
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| − | The idea is to make an organism already present in idli (<i>Lactobacillus plantarum</i>) produce vitamin B12. A close cousin of this organism, <i>L. reuteri</i> was thought to produce B12 and had the advantage to have the cobalamin biosynthesis bathway in one single stretch of DNA.
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| − | <h2 class="date">June 42nd</h2>
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| − | <b>Overexpress the B12 production pathway in <i>Lactobacillus reuteri</i></b><br>
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| − | The idea is to take an organism already present in idli (<i>Lactobacillus reuteri</i>) and to make it produce even more B12. Its pathway has been studied and we planned to metabolic engineer it as it had already been done in <i>Bacillus megaterium</i> (Biedendieck <i>et al.</i>, 2010).<br>
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| − | The problem is that <i>L. reuteri</i> actually produces pseudo-cobalamin, which has not been proven to be active in mammals (<span style="color: red">Santos reference?</span>).<br>
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| − | So instead of overproducing a potentially non-active form of cobalamin, we thought of changing the pathway in <i>L. reuteri</i> to make it produce an active form of cobalamin. Unfortunately, due to an apparent difficulty and a lack of knowledge in the community, we dropped this idea.
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| − | <hr>
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| | <a name="july" class="anchor"><h1>July</h1></a> | | <a name="july" class="anchor"><h1>July</h1></a> |
| | <h2 class="date">July 1st</h2> | | <h2 class="date">July 1st</h2> |
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| | <h2>References</h2> | | <h2>References</h2> |
| − | Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37. | + | Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.<br> |
| − | | + | B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf—br> |
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| − | <li>Toto
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| − | <li>Tutu</li>
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| − | <li>Têtê</li>
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| − | </ul></li>
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| − | </ul>
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