Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 121: | Line 121: | ||
<li>200 uL C -</li> | <li>200 uL C -</li> | ||
<li>Incubation overnight at 37°C</li> | <li>Incubation overnight at 37°C</li> | ||
+ | |||
+ | |||
<div id="notebookContent"> | <div id="notebookContent"> | ||
Line 128: | Line 130: | ||
<h2>Goal 1</h2> | <h2>Goal 1</h2> | ||
Check the transformations I've done yesterday | Check the transformations I've done yesterday | ||
+ | |||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
<ol> | <ol> | ||
Line 133: | Line 136: | ||
Transformation of my construction didn’t worked, I’ll start again all the process : PCR, purify, digest, ligate, integrate (heat shock), overnight culture. | Transformation of my construction didn’t worked, I’ll start again all the process : PCR, purify, digest, ligate, integrate (heat shock), overnight culture. | ||
</ol> | </ol> | ||
+ | |||
<h2>Goal 2</h2> | <h2>Goal 2</h2> | ||
PCR my backbone and my insert | PCR my backbone and my insert | ||
+ | |||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
<li>PCR1' = Backbone + 068 and 069 primers, before purify</li> | <li>PCR1' = Backbone + 068 and 069 primers, before purify</li> |
Revision as of 08:55, 8 September 2015