Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminB12"

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<div id="notebookContent">
 
<div id="notebookContent">
 
<a name="july" class="anchor"><h1>July</h1></a>
 
<a name="july" class="anchor"><h1>July</h1></a>
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<h2 class="date">July 1st</h2>
+
<h2 class="date">July 28th</h2>
−
Bananaaaa
+
<ul>
 +
  <li>Received <i>Propionibacterium freudenreichii subsp. shermanii</i> CIRM BIA1 from the INRA Rennes CIRM collection.</li>
 +
  <li>Made the following media:
 +
  <ul>
 +
    <li><b>MEA (waking lyophilized bacteria up), 1 L</b>
 +
      <table>1
 +
        <tr><th>Product</th><th>Quantity</th></tr>
 +
        <tr><td>BHI broth</td><td>37 g</td></tr>
 +
        <tr><td>Soja peptone</td><td>5 g</td></tr>
 +
        <tr><td>Yeast extract</td><td>5 g</td></tr>
 +
        <tr><td>Glucose</td><td>3 g</td></tr>
 +
        <tr><td>Water</td><td>Fill to 1 L</td></tr>
 +
      </table> 
 +
    </li>
 +
    <li><b>YEL (propionibacteria growth medium), 1 L</b>
 +
 
 +
    </li>
 +
  </ul>
 +
  </li>
 +
</ul>
 +
 
 
<hr>
 
<hr>
 
<h2>References</h2>
 
<h2>References</h2>

Revision as of 20:53, 9 September 2015

July

July 28th

  • Received Propionibacterium freudenreichii subsp. shermanii CIRM BIA1 from the INRA Rennes CIRM collection.
  • Made the following media:
    • MEA (waking lyophilized bacteria up), 1 L 1
      ProductQuantity
      BHI broth37 g
      Soja peptone5 g
      Yeast extract5 g
      Glucose3 g
      WaterFill to 1 L
    • YEL (propionibacteria growth medium), 1 L

References

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.
B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf