Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
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<br><br> | <br><br> | ||
<h1 class="date two">August 1st</h1> | <h1 class="date two">August 1st</h1> | ||
+ | |||
+ | <h2>Goal </h2> | ||
+ | PCR my backbone and my insert again : 1" and 2" | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | PCR then purify | ||
+ | |||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date two">August 5th</h1> | ||
+ | |||
+ | <h2>Goal </h2> | ||
+ | Ligation/transformations (insertion of FINAL block from IDT in the pSB1C3 backbone, FINAL is my Riboswitch associated with an EGFP) | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | |||
+ | <li>Used 3 different insert PCR products | ||
+ | <li>Ligation after digestion and purification of PCR2’ = 94,6ng/uL , PCR2’’= 57,5 ng/uL , PCR2’’’=83,4 ng/uL ; Backbone pSB1C3 = 37,9ng/uL</li> | ||
+ | |||
+ | <style type="text/css"> | ||
+ | .tg {border-collapse:collapse;border-spacing:0;border-color:#aabcfe;} | ||
+ | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:5px 20px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-color:#aabcfe;color:#669;background-color:#e8edff;} | ||
+ | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:5px 20px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-color:#aabcfe;color:#039;background-color:#b9c9fe;} | ||
+ | .tg .tg-c9cr{font-style:italic} | ||
+ | .tg .tg-s6z2{text-align:center} | ||
+ | .tg .tg-e3zv{font-weight:bold} | ||
+ | .tg .tg-hgcj{font-weight:bold;text-align:center} | ||
+ | </style> | ||
+ | <table class="tg"> | ||
+ | <tr> | ||
+ | <th class="tg-031e"></th> | ||
+ | <th class="tg-e3zv">'</th> | ||
+ | <th class="tg-e3zv">"</th> | ||
+ | <th class="tg-hgcj">"'</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Insert</td> | ||
+ | <td class="tg-031e">4</td> | ||
+ | <td class="tg-031e">7</td> | ||
+ | <td class="tg-s6z2">5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Backbone</td> | ||
+ | <td class="tg-031e">5</td> | ||
+ | <td class="tg-031e">5</td> | ||
+ | <td class="tg-s6z2">5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Buffer</td> | ||
+ | <td class="tg-031e">2</td> | ||
+ | <td class="tg-031e">2</td> | ||
+ | <td class="tg-s6z2">2</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Ligase</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | <td class="tg-031e">1</td> | ||
+ | <td class="tg-s6z2">1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Water</td> | ||
+ | <td class="tg-031e">8</td> | ||
+ | <td class="tg-031e">5</td> | ||
+ | <td class="tg-s6z2">7</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-c9cr">TOTAL</td> | ||
+ | <td class="tg-031e">20uL</td> | ||
+ | <td class="tg-031e">20uL</td> | ||
+ | <td class="tg-s6z2">20uL</td> | ||
+ | </tr> | ||
+ | </table> |
Revision as of 09:14, 8 September 2015