Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
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<h2>Procedure</h2> | <h2>Procedure</h2> | ||
− | <li>Used 3 different insert PCR products | + | <li>Used 3 different insert PCR products</li> |
<li>Ligation after digestion and purification of PCR2’ = 94,6ng/uL , PCR2’’= 57,5 ng/uL , PCR2’’’=83,4 ng/uL ; Backbone pSB1C3 = 37,9ng/uL</li> | <li>Ligation after digestion and purification of PCR2’ = 94,6ng/uL , PCR2’’= 57,5 ng/uL , PCR2’’’=83,4 ng/uL ; Backbone pSB1C3 = 37,9ng/uL</li> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | |||
+ | I did then a transformation using the iGEM protocol (Heat Shock) with 50uL of chemically competent cells + 3 uL of Ligation Product for each (pSB1C3-TOTAL) </li> | ||
+ | |||
+ | <li>C + : pSB1C3-RFP </li> | ||
+ | <li>C - : just competent cells </li> | ||
+ | <br> | ||
+ | <li>Plated on Cm 25 ug/mL </li> | ||
+ | |||
+ | <li>5 plates : ‘,’’,’’’,C+,C- </li> | ||
+ | |||
+ | <h2>Results</h2> | ||
+ | <li>C- : nothing</li> | ||
+ | <li>C+ : lot of red colonies</li> | ||
+ | <li>‘ : lot</li> | ||
+ | <li>‘’ : lot</li> | ||
+ | <li>‘’’ : lot</li> | ||
+ | <li>I should have done a control transformation with just the backbone</li> |
Revision as of 09:21, 8 September 2015