Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 237: | Line 237: | ||
<li>‘’’ : lot</li> | <li>‘’’ : lot</li> | ||
<li>I should have done a control transformation with just the backbone</li> | <li>I should have done a control transformation with just the backbone</li> | ||
+ | |||
+ | <br><br> | ||
+ | <h1 class="date two">August 6th</h1> | ||
+ | |||
+ | <h2>Goal 1 </h2> | ||
+ | Cultivate my transformants | ||
+ | |||
+ | <h2>Procedure</h2> | ||
+ | <li>There is red and white transformant colonies, all resistant to Cm 25</li> | ||
+ | <li>I selected some white colonies overnight culture </li> | ||
+ | <li>On plate LB Cm —> it grew (some red, maybe contamination)</li> | ||
+ | <li>On broth LB Cm —> it grew (some red, maybe contamination)</li> | ||
+ | <li>Inoculated at 4:00 pm in LB Cm 25 bacteria from a culture ( red —> RFP ) with different AdoCbl concentrations in order to detect the minimal amount of B12 viable for e.coli : | ||
+ | 5; 10; 20; 40; 80; 160 ng/mL</li> | ||
+ | |||
+ | <h2>Goal 2 </h2> | ||
+ | PCR the pSB1C3 backbone and my FINAL riboswitch construction |
Revision as of 15:26, 8 September 2015