Revision as of 20:57, 9 September 2015

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

July
August
September

July

July 28th

Received Propionibacterium freudenreichii subsp. shermanii CIRM BIA1 from the INRA Rennes CIRM collection.
Made the following media:
- MEA (waking lyophilized bacteria up), 1 L
  
  Product Quantity
  
  BHI broth 37 g
  
  Soja peptone 5 g
  
  Yeast extract 5 g
  
  Glucose 3 g
  
  Water Fill to 1 L
- YEL (propionibacteria growth medium), 1 L
  
  Product Quantity
  
  Sodium lactate (60% syrup) 21.4 g
  
  Tryptone 10 g
  
  Yeast extract 10 g
  
  K₂HPO₄, 3 H₂O 328 mg
  
  MnSO₄, H₂O 56 mg
  
  Water Fill to 1 L

References

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.
B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf

@@ Line 21: / Line 21: @@
    <ul>
      <li><b>MEA (waking lyophilized bacteria up), 1 L</b>
-       <table>1
+       <table>
          <tr><th>Product</th><th>Quantity</th></tr>
          <tr><td>BHI broth</td><td>37 g</td></tr>
@@ Line 31: / Line 31: @@
      </li>
      <li><b>YEL (propionibacteria growth medium), 1 L</b>
+      <table>
+        <tr><th>Product</th><th>Quantity</th></tr>
+        <tr><td>Sodium lactate (60% syrup)</td><td>21.4 g</td></tr>
+        <tr><td>Tryptone</td><td>10 g</td></tr>
+        <tr><td>Yeast extract</td><td>10 g</td></tr>
+        <tr><td>K<sub>2</sub>HPO<sub>4</sub>, 3 H<sub>2</sub>O</td><td>328 mg</td></tr>
+        <tr><td>MnSO<sub>4</sub>, H<sub>2</sub>O</td><td>56 mg</td></tr>
+        <tr><td>Water</td><td>Fill to 1 L</td></tr>
+      </table>
      </li>
    </ul>

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