Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminB12"
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</ul> | </ul> | ||
</li> | </li> | ||
| + | <li>Successfully grew <i>P. freudenreichii</i> in MEA at 30°C, in aerobic conditions.</li> | ||
| + | </ul> | ||
| + | |||
| + | <h2 class="date">July 30th</h2> | ||
| + | <img style="float:right" src="https://static.igem.org/mediawiki/2015/f/f7/PB_B12_Result_plates.png" width="400px"> | ||
| + | <ul> | ||
| + | <li>Tested the YEL+agar medium with the following strains: | ||
| + | <ul> | ||
| + | <li><i>P. freudenreichii</i></li> | ||
| + | <li><i>Lactobacillus lactis</i></li> | ||
| + | <li><i>E. coli</i></li> | ||
| + | <li><i>Lactobacillus plantarum</i></li> | ||
| + | <li><i>Saccharomyces cerevisiae</i></li> | ||
| + | </ul></li> | ||
| + | <li>Result (after 24 hours): | ||
| + | |||
</ul> | </ul> | ||
Revision as of 21:16, 9 September 2015
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms July
July 28th
- Received Propionibacterium freudenreichii subsp. shermanii CIRM BIA1 from the INRA Rennes CIRM collection.
- Made the following media:
- MEA (waking lyophilized bacteria up), 1 L
Product Quantity BHI broth 37 g Soja peptone 5 g Yeast extract 5 g Glucose 3 g Water Fill to 1 L - YEL (propionibacteria growth medium), 1 L
Product Quantity Sodium lactate (60% syrup) 21.4 g Tryptone 10 g Yeast extract 10 g K2HPO4, 3 H2O 328 mg MnSO4, H2O 56 mg Water Fill to 1 L
- MEA (waking lyophilized bacteria up), 1 L
- Successfully grew P. freudenreichii in MEA at 30°C, in aerobic conditions.
July 30th
- Tested the YEL+agar medium with the following strains:
- P. freudenreichii
- Lactobacillus lactis
- E. coli
- Lactobacillus plantarum
- Saccharomyces cerevisiae
- Result (after 24 hours):
References
Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.
B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf
