Difference between revisions of "Team:Exeter/Future"
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Further characterisation of our parts is essential to the progress of our project, as a starting point we designed our synthetic toehold switch with GFP attached; this was developed with the intention of obtaining quantitative data for our modelling team. Since we aimed to make our test as low tech as possible we decided to design several more toeholds, each with a different chromoprotein that would be visible to the naked eye. This switching of indicator within our toehold is a relatively simple process which demonstrates the flexibility and adaptability of our synthetic toehold. | Further characterisation of our parts is essential to the progress of our project, as a starting point we designed our synthetic toehold switch with GFP attached; this was developed with the intention of obtaining quantitative data for our modelling team. Since we aimed to make our test as low tech as possible we decided to design several more toeholds, each with a different chromoprotein that would be visible to the naked eye. This switching of indicator within our toehold is a relatively simple process which demonstrates the flexibility and adaptability of our synthetic toehold. | ||
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The different indicators we used to alter our synthetic toehold switches were the chromoproteins - eforRed, aeBlue, amajlime and tinsel purple but also RFP and Luciferase. Although these were the indicators we worked into our sequences, they were not the chromoproteins we further characterised for the Registry. Since our test was designed to be cell free this was the logical route for further characterisation. The biobricks we further characterised are: | The different indicators we used to alter our synthetic toehold switches were the chromoproteins - eforRed, aeBlue, amajlime and tinsel purple but also RFP and Luciferase. Although these were the indicators we worked into our sequences, they were not the chromoproteins we further characterised for the Registry. Since our test was designed to be cell free this was the logical route for further characterisation. The biobricks we further characterised are: | ||
Revision as of 21:26, 9 September 2015
Future Characterisation
Further characterisation of our parts is essential to the progress of our project, as a starting point we designed our synthetic toehold switch with GFP attached; this was developed with the intention of obtaining quantitative data for our modelling team. Since we aimed to make our test as low tech as possible we decided to design several more toeholds, each with a different chromoprotein that would be visible to the naked eye. This switching of indicator within our toehold is a relatively simple process which demonstrates the flexibility and adaptability of our synthetic toehold.
The different indicators we used to alter our synthetic toehold switches were the chromoproteins - eforRed, aeBlue, amajlime and tinsel purple but also RFP and Luciferase. Although these were the indicators we worked into our sequences, they were not the chromoproteins we further characterised for the Registry. Since our test was designed to be cell free this was the logical route for further characterisation. The biobricks we further characterised are: BBa_K1073022 (eforRed with J23100 promoter) BBa_K1073020 (aeBlue with J23100 promoter) BBa_K1431814 (amajLime with J23100 promoter) BBa_K1431812 (amilCP with J23100 promoter) BBa_ K1033923 (spisPink with J23110 promoter) Potential characterisation would make it easy for academics and researchers to use our switches in experiments as they can be tailored to the indicator they would like to use or the equipment they have available to visualise the indicator.
This is a link --->Future Applications
Coming Soon!
Future Optimisations
Coming Soon!