Difference between revisions of "Team:Exeter/Protocols"
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><em>E.coli</em> transformation.</a></li> | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/7/77/Exeter_NEB_transformation_SOP.pdf"><em>E.coli</em> transformation.</a></li> | ||
<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/e/e6/Exeter_gBlock_HiFi_SOP.pdf">gBlock Prep. and HiFi assembly.</a></li> | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/e/e6/Exeter_gBlock_HiFi_SOP.pdf">gBlock Prep. and HiFi assembly.</a></li> | ||
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<li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf">Solid agar recipe for agar plates.</a></li> | <li><a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf">Solid agar recipe for agar plates.</a></li> | ||
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Revision as of 14:29, 13 September 2015
Protocols
Use the mouse or touch screen to scroll through our SOPs. Click on an image to bring up the corresponding protocol.
- E.coli transformation.
- gBlock Prep. and HiFi assembly.
- Competent cells preparation.
- Protein extraction (BugBuster).
- LB agar broth recipe.
- Agarose gel recipe.
- Running gel electrophoresis.
- Using the autoclave.
- DNA restriction digest.
- Thermo scientific FastDigest.
- DNA gel extraction.
- Cell glycerol stocks.
- Promega DNA ligation.
- Qiagen DNA miniprep.
- Liquid cell cultures.
- MgCl2 and CaCl2 recipes.
- Sterile 40% glycerol recipe.
- Qubit for DNA concentration.
- Dried DNA rehydration.
- Promega S30 cell free kit.
- Solid agar recipe for agar plates.