Difference between revisions of "Team:Exeter/Results"

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<div class="container">
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<div id="container">
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<h1>Experiments &amp; Protocols</h1>
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<div id="toehold_experiments">
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<h2>Toehold experiments</h2>
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<div id="in_silico">
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<h4><em>In silico</em> testing:</h4>
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</div>
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<div id="validation">
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<h4>Experimental validation of GreenFET1J (<a href="https://2015.igem.org/Team:Exeter/Parts#toeholds">BBa_K1586000</a>):</h4>
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</div>
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</div>
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<div id="further_characterisation">
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<h2>Further characterisation:</h2>
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<p>
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As has been stated previously, we wish our diagnostic test to be carried out in the field with little technical equipment/training, therefore the visualisation of the result must be simple. In order to do this, we decided that a colour change would be best, and that the expression of chromoproteins on activation of the toehold would be a good way to achieve this. In order to better inform ourselves and the model, and also to generate data which would be useful for testing toeholds with chromoproteins as the protein coding regions, we decided to further characterise some of the chromoproteins in the iGEM registry; <a href="http://parts.igem.org/Part:BBa_K1431814">amajLime</a>, <a href="http://parts.igem.org/Part:BBa_K1073020">aeBlue</a>, and <a href="http://parts.igem.org/Part:BBa_K1073022">eforRed</a>.
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</p>
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<h4>Absorbance spectra:</h4>
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<h4>Standard curve - concentration vs. OD:</h4>
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</div>
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Revision as of 19:24, 18 September 2015

Experiments & Protocols

Toehold experiments

In silico testing:

Experimental validation of GreenFET1J (BBa_K1586000):

Further characterisation:

As has been stated previously, we wish our diagnostic test to be carried out in the field with little technical equipment/training, therefore the visualisation of the result must be simple. In order to do this, we decided that a colour change would be best, and that the expression of chromoproteins on activation of the toehold would be a good way to achieve this. In order to better inform ourselves and the model, and also to generate data which would be useful for testing toeholds with chromoproteins as the protein coding regions, we decided to further characterise some of the chromoproteins in the iGEM registry; amajLime, aeBlue, and eforRed.

Absorbance spectra:

Standard curve - concentration vs. OD:

  • Contact us:
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