Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 1,233: | Line 1,233: | ||
Ligation | Ligation | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
+ | <style type="text/css"> | ||
+ | .tg {border-collapse:collapse;border-spacing:0;} | ||
+ | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | </style> | ||
+ | <table class="tg"> | ||
+ | <tr> | ||
+ | <th class="tg-031e"></th> | ||
+ | <th class="tg-031e">La (F2d’-LP1d’)</th> | ||
+ | <th class="tg-031e">La c</th> | ||
+ | <th class="tg-031e">Lb (F3d’-LP3d’)</th> | ||
+ | <th class="tg-031e">Lb c</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">F2dp’</td> | ||
+ | <td class="tg-031e">6uL</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP1dp’</td> | ||
+ | <td class="tg-031e">7uL</td> | ||
+ | <td class="tg-031e">7uL</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">F3dp’</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e">15uL</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">LP3dp’</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Ligase</td> | ||
+ | <td class="tg-031e">1uL</td> | ||
+ | <td class="tg-031e">1uL</td> | ||
+ | <td class="tg-031e">1uL</td> | ||
+ | <td class="tg-031e">1uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">L buffer</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Water</td> | ||
+ | <td class="tg-031e">4uL</td> | ||
+ | <td class="tg-031e">10uL</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e">15uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">TOTAL</td> | ||
+ | <td class="tg-031e">20uL</td> | ||
+ | <td class="tg-031e">20uL</td> | ||
+ | <td class="tg-031e">20uL</td> | ||
+ | <td class="tg-031e">20uL</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | Transformed 5uL in 50 uL of competent cells - Heat shock |
Revision as of 10:44, 16 September 2015