Difference between revisions of "Team:Paris Bettencourt/Project/VitaminB2"
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<p>Genetic sequences of <i>Bacillus subtilis</i> four enzymes were codon optimized for <i>Lactobacillus plantarum</i> on IDT website tool.<br> | <p>Genetic sequences of <i>Bacillus subtilis</i> four enzymes were codon optimized for <i>Lactobacillus plantarum</i> on IDT website tool.<br> | ||
− | The enzyme was expressed by a promoter rather than a single operon expression to allow a precise tuning of the gene transcription. The GTP cyclohydrolase | + | The enzyme was expressed by a promoter rather than a single operon expression to allow a precise tuning of the gene transcription. Two bottlenecks were identified in the pathway (M. Birkenmeier, Biotech. Lett., 2014). The first bottleneck correspond to the GTP cyclohydrolase activity of RibA. Overcoming the first bottleneck creates a second bottleneck corresponding to RibT's lumazine synthase activity.<br> |
+ | To promote a differential expression of the four genes, we used the two synthetic <i>Lactobacillus</i> promoters p25 and p48 (respectively medium and strong expressing promoters) (I. Rud, Microbiology, 2006).<br> | ||
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RibD: Pyrimidine deaminase/reductase<br> | RibD: Pyrimidine deaminase/reductase<br> | ||
RibE: Riboflavin synthase, beta-chain<br> | RibE: Riboflavin synthase, beta-chain<br> | ||
− | RibT: Riboflavin synthase, alpha-chain<br> | + | RibT: lumazine synthase, (Riboflavin synthase, alpha-chain)<br> |
Revision as of 14:33, 18 September 2015