Difference between revisions of "Team:Hamburg/Parts"
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<p>Two <a href="https://2015.igem.org/Team:Hamburg/Basic_Part">basic parts</a> contain the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860700">miRNA</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860701">GroEL</a>. Two <a href="https://2015.igem.org/Team:Hamburg/Composite_Part">composite parts</a> contain a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860702">constitutive promoter + miRNA</a> system (where the constitutive promoter replaces the light-inducible promoter as intermediate step) and a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860703">GroEL + blue pigment</a> system (where the blue pigment replaces the cell lysis protein PezT for now). Further steps would be to design a <i>light promoter + miRNA</i> biobrick and a <i>GroEL + PezT</i> biobrick, to then combine them by 3A assembly for our ultimate goal.</p> | <p>Two <a href="https://2015.igem.org/Team:Hamburg/Basic_Part">basic parts</a> contain the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860700">miRNA</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860701">GroEL</a>. Two <a href="https://2015.igem.org/Team:Hamburg/Composite_Part">composite parts</a> contain a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860702">constitutive promoter + miRNA</a> system (where the constitutive promoter replaces the light-inducible promoter as intermediate step) and a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1860703">GroEL + blue pigment</a> system (where the blue pigment replaces the cell lysis protein PezT for now). Further steps would be to design a <i>light promoter + miRNA</i> biobrick and a <i>GroEL + PezT</i> biobrick, to then combine them by 3A assembly for our ultimate goal.</p> | ||
+ | |||
+ | <style> | ||
+ | #fig2 img{ | ||
+ | width: 75% | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <figure id="fig2"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/8/89/Hamburg_mainpic.jpg" alt="Our concept" /> | ||
+ | <figcaption>This figure delineates the basic steps of our ultimate goal.</figcaption> | ||
+ | </figure> | ||
Revision as of 18:21, 18 September 2015
Parts
Our ultimate goal was to create a Biobrick enabling light-inducible miRNA2911 production and heat-inducible cell lysis for the release afterwards in one plasmid (see figure). Despite not reaching this goal so far, we completed important intermediate steps successfully heading toward it.
![](https://static.igem.org/mediawiki/2015/b/b6/Hamburg_parts4.jpg)
Two basic parts contain the miRNA and GroEL. Two composite parts contain a constitutive promoter + miRNA system (where the constitutive promoter replaces the light-inducible promoter as intermediate step) and a GroEL + blue pigment system (where the blue pigment replaces the cell lysis protein PezT for now). Further steps would be to design a light promoter + miRNA biobrick and a GroEL + PezT biobrick, to then combine them by 3A assembly for our ultimate goal.
![Our concept](https://static.igem.org/mediawiki/2015/8/89/Hamburg_mainpic.jpg)
Team Parts
<groupparts>iGEM015 Hamburg</groupparts>