Difference between revisions of "Team:Paris Bettencourt/Design"
CeliaFoulon (Talk | contribs) |
|||
Line 45: | Line 45: | ||
<div class="column-left" align="justify"> | <div class="column-left" align="justify"> | ||
Our project is designed for poor regions: the cheaper our product, the more people it can reach. Starting from | Our project is designed for poor regions: the cheaper our product, the more people it can reach. Starting from | ||
− | this point, we | + | this point, we didn't want to build labs in India, buy media and grow our strains in expensive structures. |
− | Therefore, we had to think of a cheap solution to grow the strains in a homemade media, made of | + | Therefore, we had to think of a cheap solution to grow the strains in a homemade media, made of common ingredients, cheap and readily available. <br> |
− | To avoid expensive and time-consuming treatment, an edible medium is sought. It could | + | To avoid expensive and time-consuming treatment, an edible medium is sought. It could be directly used to |
cook either our distribution product or the Idli directly. The process would hence be quite simple and | cook either our distribution product or the Idli directly. The process would hence be quite simple and | ||
accessible to the population.<br> | accessible to the population.<br> | ||
Line 55: | Line 55: | ||
After some research, we selected 4 different homemade media to test with our 3 strains: | After some research, we selected 4 different homemade media to test with our 3 strains: | ||
<ul> | <ul> | ||
− | <li>Water from the cooking of potato</li> | + | <li>Water from the cooking of potato (potatoes cooked in 2L of water for 20min)</li> |
<li>Water from the cooking of potato+sugar(10%)</li> | <li>Water from the cooking of potato+sugar(10%)</li> | ||
− | <li>Water from the cooking of rice</li> | + | <li>Water from the cooking of rice (200ml of rice cooked in 2L of water for 10min) </li> |
<li>Water from the cooking of rice+milk(10%)</li> | <li>Water from the cooking of rice+milk(10%)</li> | ||
</ul> | </ul> | ||
− | <br>For each strain, we | + | <br>We also wanted to see if the concentration in potato had an influence so for each media made out of potato water, we tried two different concentration of potatoes: 190g/L (named ???????) and 250g/L (named ???????). |
+ | <br>For each strain, we compared the growth of the strain in the homemade media with the growth in its | ||
"normal" media (respectively YPD, M17+Glucose 1% and YEL). | "normal" media (respectively YPD, M17+Glucose 1% and YEL). | ||
</div> | </div> | ||
Line 77: | Line 78: | ||
We could have just lyophilized the strains but our goal is to design something cheap and easy to do for the | We could have just lyophilized the strains but our goal is to design something cheap and easy to do for the | ||
locals, using only ingredients they have access to and not time consuming.<br> | locals, using only ingredients they have access to and not time consuming.<br> | ||
− | We first tried to | + | We first tried to make different kind of powders, but none of it was satisfying. Powders appeared not to be very convenient. |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
<div class="column-right" align="justify"> | <div class="column-right" align="justify"> | ||
− | + | We realised a powder wasn't the best way to distribute our strains. We found better to make portions, easy to pack, with the possibility to pack several portions together. Portions must be easy to stock.<br> | |
− | + | ||
− | + | ||
The most efficient and ergonomic shape appeared to be a cube. Moreover, the cube will mainly be added to | The most efficient and ergonomic shape appeared to be a cube. Moreover, the cube will mainly be added to | ||
Idli, made of rice, so rice flour seemed to be a logical ingredient, consonant with the dish, common in | Idli, made of rice, so rice flour seemed to be a logical ingredient, consonant with the dish, common in | ||
India and cheap.<br> | India and cheap.<br> | ||
− | Little by little, we succeeded to design an easy recipe, to cook small cubes made of rice flour and water. | + | Little by little, we succeeded to design an easy recipe, to cook small cubes made of rice flour and water: the VitaCubes. |
− | The idea was also to be flexible to every | + | The idea was also to be flexible to every VitaCube maker means, therefore the recipe is not very strict and can |
be adapted to what the people have available.<br> | be adapted to what the people have available.<br> | ||
</div> | </div> | ||
Line 117: | Line 93: | ||
<div class="column-left" align="justify"> | <div class="column-left" align="justify"> | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
Line 142: | Line 103: | ||
<div class="column-left" align="justify"> | <div class="column-left" align="justify"> | ||
− | Now that we have found a | + | Now that we have found a convenient distribution mean, we need to be sure that it keeps our strains alive and to know how long it can be stored.<br> |
− | We conducted several survival test on the | + | We conducted several survival test on the VitaCubes, using <i>S.cerevisiae</i> mCherry and <i>L.</i> lactis. To make these test, we were plating the liquid solution containing the strains before adding it to the flour, in order to know how many cells we put in the VitaCube. After a define period of drying, we immerse the VitaCube in 10ml of osmosed water and plated the solution obtained to count the numbers of cells that survived in the cube.<br> At first, we obtained very different results, even if the cubes were all plated after one day of drying. We realised the time of ??? of the cube in the water had an influence over the survival rate: the longer it was, the bigger was the survival rate. This is due to the revival of cells once back in water.<br> |
+ | Therefore, we designed a one week experiment: we made plenty of VitaCubes on the same day, and then each day, we plated one cube. Each day, we respected the very same conditions, every step was timed.<br> | ||
+ | Graphs!!!!!!!!!! <br> | ||
+ | |||
+ | We can see that the yeasts are very resistant and have a very high survival rate, decreasing very slowly over time. The survival rate is even higher than 1 because yeasts | ||
</div> | </div> | ||
<div class="column-right" align="justify"> | <div class="column-right" align="justify"> |
Revision as of 01:16, 19 September 2015