Difference between revisions of "Team:Paris Bettencourt/Project/Manufacturing"
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<br>We also wanted to see if the concentration in potato had an influence so for each media made out of potato water, we tried two different concentrations of potato: 190g/L (named potato dilute) and 250g/L (named potato concentrated). | <br>We also wanted to see if the concentration in potato had an influence so for each media made out of potato water, we tried two different concentrations of potato: 190g/L (named potato dilute) and 250g/L (named potato concentrated). | ||
<br>For each strain, we compared the growth of the strain in the homemade media with the growth in its | <br>For each strain, we compared the growth of the strain in the homemade media with the growth in its | ||
− | "normal" media (respectively YPD, M17+Glucose 1% and YEL).<br> The results can be seen | + | "normal" media (respectively YPD, M17+Glucose 1% and YEL).<br> The results can be seen in Figure 1. |
</div> <div style="clear:both"> | </div> <div style="clear:both"> | ||
<div class="column-left" align="justify"> | <div class="column-left" align="justify"> | ||
− | + | As we can see in the first figure, the strains are growing in each media. The rice media is the best one, giving even better result than the normal media for <i>Lactococcus</i> Lactis and <i>Propionibacterium</i> freudenreichii. We couldn't show that neither the concentration of potato nor the addition of sugar had any influence on the growth of our strains.<br> | |
<b>So finally, we shown that our strains could be grown in potato water and rice water.</b> | <b>So finally, we shown that our strains could be grown in potato water and rice water.</b> | ||
</div> | </div> | ||
<div class="column-right" align="justify"> <br> | <div class="column-right" align="justify"> <br> | ||
<img src="https://static.igem.org/mediawiki/2015/3/3c/PBmediafig.png" alt="Graph"><br> | <img src="https://static.igem.org/mediawiki/2015/3/3c/PBmediafig.png" alt="Graph"><br> | ||
− | + | <b>Figure 1</b> | |
</div> <div style="clear:both"> | </div> <div style="clear:both"> | ||
<br><br> | <br><br> | ||
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Therefore, we designed a one week experiment: we made plenty of VitaCubes on the same day, and then each day, we plated one cube. Each day, we respected the very same conditions, every step was timed.<br> | Therefore, we designed a one week experiment: we made plenty of VitaCubes on the same day, and then each day, we plated one cube. Each day, we respected the very same conditions, every step was timed.<br> | ||
<img src="https://static.igem.org/mediawiki/2015/6/6f/PBScsurvivalrate.jpeg" alt="Graph"> <br> | <img src="https://static.igem.org/mediawiki/2015/6/6f/PBScsurvivalrate.jpeg" alt="Graph"> <br> | ||
− | + | <b>Figure 2</b><br> | |
<img src="https://static.igem.org/mediawiki/2015/0/0e/PBlactococcussurvival.jpeg" alt="Graph"><br> | <img src="https://static.igem.org/mediawiki/2015/0/0e/PBlactococcussurvival.jpeg" alt="Graph"><br> | ||
− | + | <b>Figure 3</b><br> | |
On graph 2, we can see that the yeasts are very resistant and have a very high survival rate, decreasing very slowly over time. The survival rate is even higher than 1 because yeasts are growing very fast, so in 20 minutes in the water, they divide. In conclusion, the yeasts can be stored more than one week in a VitaCube.<br> | On graph 2, we can see that the yeasts are very resistant and have a very high survival rate, decreasing very slowly over time. The survival rate is even higher than 1 because yeasts are growing very fast, so in 20 minutes in the water, they divide. In conclusion, the yeasts can be stored more than one week in a VitaCube.<br> | ||
On graph 3, we can see that <i>L.</i> lactis survival rate is low since the first day and is decreasing very quickly every day. After 4 days of drying, the survival rate started to be very very low. Nevertheless, there are still around 10<sup>5</sup> cells in a VitaCube after one week, each is far enough to make a culture from it.<br> | On graph 3, we can see that <i>L.</i> lactis survival rate is low since the first day and is decreasing very quickly every day. After 4 days of drying, the survival rate started to be very very low. Nevertheless, there are still around 10<sup>5</sup> cells in a VitaCube after one week, each is far enough to make a culture from it.<br> |
Revision as of 16:20, 15 October 2015