Difference between revisions of "Team:Paris Bettencourt/Project/VitaminB2"
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From the standard curve, we derived a linear equation mapping riboflavin concentration to fluorescence (fluorescence = 16344*x + 418).<br> | From the standard curve, we derived a linear equation mapping riboflavin concentration to fluorescence (fluorescence = 16344*x + 418).<br> | ||
− | <img width=" | + | <img width="500px" src="https://static.igem.org/mediawiki/2015/8/80/PB_overproduction_riboflavin.png"><br> |
<b>Figure 6: </b><i>Over-production of riboflavin by E.coli </i><br><br> | <b>Figure 6: </b><i>Over-production of riboflavin by E.coli </i><br><br> | ||
<i>E. coli</i> strains over-produce riboflavin up to 6µg/mL of media.<br><br> | <i>E. coli</i> strains over-produce riboflavin up to 6µg/mL of media.<br><br> | ||
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We characterized the functioning of promoter p48 in <i>E. coli</i> and BioBrick it (<a href="http://parts.igem.org/Part:BBa_K1678004">BBa_K1678004</a>).<br> | We characterized the functioning of promoter p48 in <i>E. coli</i> and BioBrick it (<a href="http://parts.igem.org/Part:BBa_K1678004">BBa_K1678004</a>).<br> | ||
Activity of p48 was shown by the expression of the fluorescent protein mCerulean.<br> | Activity of p48 was shown by the expression of the fluorescent protein mCerulean.<br> | ||
− | <img width=" | + | <img width="400px" src="https://static.igem.org/mediawiki/parts/b/bb/BB_fluorescence_chart_BB_04.png"><br> |
<b>Figure 8: </b><i>Fluorescence of mCerulean promoted by p48</i><br><br> | <b>Figure 8: </b><i>Fluorescence of mCerulean promoted by p48</i><br><br> | ||
Revision as of 23:14, 19 November 2015