Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminB12"

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<h3>June</h3>
 
<h3>June</h3>
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Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?  
+
Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?<br><br>
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<b>Overexpress the B12 production pathway in <i>Lactobacillus reuteri</i></b>
+
 
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The idea is to take an organism already present in idli (<i>Lactobacillus reuteri</i>) and to make it produce even more B12. Its pathway has been studied and we planned to metabolic engineer it as it had already been done in <i>Bacillus megaterium</i> (Biedendieck <i>et al.</i>, 2010).  
+
<b>Overexpress the B12 production pathway in <i>Lactobacillus reuteri</i></b><br>
 +
The idea is to take an organism already present in idli (<i>Lactobacillus reuteri</i>) and to make it produce even more B12. Its pathway has been studied and we planned to metabolic engineer it as it had already been done in <i>Bacillus megaterium</i> (Biedendieck <i>et al.</i>, 2010).<br>
 +
The problem is that <i>L. reuteri</i> actually produces pseudo-cobalamin, which has not been proven to be active in mammals (<span style="color: red">Santos reference?</span>)
  
  

Revision as of 17:05, 12 August 2015

June

Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?

Overexpress the B12 production pathway in Lactobacillus reuteri
The idea is to take an organism already present in idli (Lactobacillus reuteri) and to make it produce even more B12. Its pathway has been studied and we planned to metabolic engineer it as it had already been done in Bacillus megaterium (Biedendieck et al., 2010).
The problem is that L. reuteri actually produces pseudo-cobalamin, which has not been proven to be active in mammals (Santos reference?)

July

References

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.