Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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Beginning of the manufacturing project! Let's do it!<br> | Beginning of the manufacturing project! Let's do it!<br> | ||
<ul> | <ul> | ||
− | <li>Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL</li> | + | <li>Choice of the strain of yeast: Sc. M Cherry, wich is resistant to geneticin at a concentration of 200µL/mL, and produce RFP</li> |
</ul> <br> | </ul> <br> | ||
We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment: | We will have to calculate a survival rate so we have to be able to know how many cells we have in the first place for each experiment: | ||
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<ul> | <ul> | ||
<li>Centrifuge the culture tube</li> | <li>Centrifuge the culture tube</li> | ||
− | <li>Throw the media away | + | <li>Throw the media away</li> |
− | <li> | + | <li>Add a very small amount of water to detach the yeasts from the tube easily</li> |
<li>Spread on aluminium</li> | <li>Spread on aluminium</li> | ||
<li>Let it dry for 2 hours</li> | <li>Let it dry for 2 hours</li> | ||
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</ul> | </ul> | ||
<i>Results:</i><br> | <i>Results:</i><br> | ||
− | The aluminium is not a good idea, the yeasts stick to it and it gets destroyed when we scratch. We have to find another drying paper.<br> | + | The aluminium is not a good idea, the yeasts stick to it and it gets destroyed when we scratch.<br> |
− | + | We have to find another drying paper.<br> | |
+ | <br> | ||
<b>Wednesday, Jul. 29</b><br> | <b>Wednesday, Jul. 29</b><br> | ||
− | Result of the first OD test:<br> | + | <i>Result of the first OD test:</i><br> |
− | The 10mL | + | The 10mL solution of yeasts/water had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies.<br> |
so '''1 OD = 1,4x10^7 cells/mL'''<br> | so '''1 OD = 1,4x10^7 cells/mL'''<br> | ||
We launched a similar experience for more repetitions.<br> | We launched a similar experience for more repetitions.<br> | ||
+ | <br> | ||
+ | <b>Thursday, Jul. 30</b><br> | ||
+ | <i>Results of the drying:</i><br> | ||
+ | Yeasts grew in the plate. We can check that they produce RFP, it's Sc. M Cherry who survived.<br> | ||
+ | Let's repeat the experience but drying the yeasts on a plastic dish.<br> | ||
+ | This time, we took the OD and plated a known quantity of the obtained powder to be able to calculate the survival rate.<br> | ||
+ | <br> | ||
+ | <b>Friday, Jul. 31</b><br> | ||
+ | <i>Result of the second OD test:</i><br> | ||
+ | <br> | ||
+ | <b>Monday, Aug. 3</b><br> | ||
<h1>eiuaeuaie</h1> | <h1>eiuaeuaie</h1> |
Revision as of 12:15, 13 August 2015