Difference between revisions of "Team:Paris Bettencourt/Protocols/Ligation"

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     <td><b>Ligation Protocol</b><br>
 
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Revision as of 17:35, 14 August 2015

Ligation Protocol
  • Mix the following
    • vector 100ng
    • insert 300ng
    • T4 DNA ligase buffer 10X
    • T4 DNA ligase
    • up to 20µL of water
  • incubate 30 to 40 minutes at room temperature