Difference between revisions of "Team:Hamburg/Modeling/Networks"
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<h2>Genetic toggle switch for PezT expression</h2> | <h2>Genetic toggle switch for PezT expression</h2> | ||
| − | <p>Our GroEL- | + | <p>Our GroEL-PezT biobrick shall enable heat-inducible cell lysis. Conducting heat induction would release our miRNA2911 production which was triggered by light before. One major problem here is an activation of the GroEL promotor and thus PetZ caused cell lysis at normal temperature; therefore not reaching sufficient miRNA product. To stabilise our cell lysis event, we can employ a gene regulatory network called the genetic toggle switch [1]. It consists of a double negative feedback loop leading to a bistability network with memory function. This means the following:</p> |
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| + | <li></li> | ||
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Revision as of 19:49, 25 August 2015
Genetic toggle switch for PezT expression
Our GroEL-PezT biobrick shall enable heat-inducible cell lysis. Conducting heat induction would release our miRNA2911 production which was triggered by light before. One major problem here is an activation of the GroEL promotor and thus PetZ caused cell lysis at normal temperature; therefore not reaching sufficient miRNA product. To stabilise our cell lysis event, we can employ a gene regulatory network called the genetic toggle switch [1]. It consists of a double negative feedback loop leading to a bistability network with memory function. This means the following:
DLE analysis
- SimRNA: a coarse-grained method for RNA folding simulations and 3D structure prediction. Michał J. Boniecki, Grzegorz Łach, Konrad Tomala, Wayne Dawson, Paweł Łukasz, Tomasz Sołtysiński, Kristian M. Rother, and Janusz M. Bujnicki. (to be published).
- Humphrey, W., Dalke, A. and Schulten, K., "VMD - Visual Molecular Dynamics", J. Molec. Graphics, 1996, vol. 14, pp. 33-38.