Difference between revisions of "Team:Hamburg/Modeling/Networks"
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<p>Our GroEL-PezT biobrick shall enable heat-inducible cell lysis. Conducting heat induction would release our miRNA2911 production which was triggered by light before. One major problem here is an activation of the GroEL promotor and thus PetZ caused cell lysis at normal temperature; therefore not reaching sufficient miRNA product. To stabilise our cell lysis event, we can employ a gene regulatory network called the genetic toggle switch [1]. It consists of a double negative feedback loop leading to a bistability network with memory function. This means the following:</p> | <p>Our GroEL-PezT biobrick shall enable heat-inducible cell lysis. Conducting heat induction would release our miRNA2911 production which was triggered by light before. One major problem here is an activation of the GroEL promotor and thus PetZ caused cell lysis at normal temperature; therefore not reaching sufficient miRNA product. To stabilise our cell lysis event, we can employ a gene regulatory network called the genetic toggle switch [1]. It consists of a double negative feedback loop leading to a bistability network with memory function. This means the following:</p> | ||
<ul> | <ul> | ||
| − | <li></li> | + | <li>the network can only attain two possible steady states (concentrations of all components): PezT-expression-state versus no-PezT-expression state</li> |
| − | <li></li> | + | <li>when one of the two possible steady state is once reached, this network state is stable (memory function)</li> |
| − | <li></li> | + | <li>each state is induced by a certain set of start conditions; it is also possible to switch the state</li> |
</ul> | </ul> | ||
Revision as of 19:56, 25 August 2015
Genetic toggle switch for PezT expression
Our GroEL-PezT biobrick shall enable heat-inducible cell lysis. Conducting heat induction would release our miRNA2911 production which was triggered by light before. One major problem here is an activation of the GroEL promotor and thus PetZ caused cell lysis at normal temperature; therefore not reaching sufficient miRNA product. To stabilise our cell lysis event, we can employ a gene regulatory network called the genetic toggle switch [1]. It consists of a double negative feedback loop leading to a bistability network with memory function. This means the following:
- the network can only attain two possible steady states (concentrations of all components): PezT-expression-state versus no-PezT-expression state
- when one of the two possible steady state is once reached, this network state is stable (memory function)
- each state is induced by a certain set of start conditions; it is also possible to switch the state
DLE analysis
- SimRNA: a coarse-grained method for RNA folding simulations and 3D structure prediction. Michał J. Boniecki, Grzegorz Łach, Konrad Tomala, Wayne Dawson, Paweł Łukasz, Tomasz Sołtysiński, Kristian M. Rother, and Janusz M. Bujnicki. (to be published).
- Humphrey, W., Dalke, A. and Schulten, K., "VMD - Visual Molecular Dynamics", J. Molec. Graphics, 1996, vol. 14, pp. 33-38.