Difference between revisions of "Team:Paris Bettencourt/Protocols/titration-acid-phytic"

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           <ul>
 
           <ul>
 
           <li><i>Trichloroacetic acid  (50% w/v) : 100mL</i></li>
 
           <li><i>Trichloroacetic acid  (50% w/v) : 100mL</i></li>
−
           <li>50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)</li>
+
           <li>Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)</li>
−
           <li></li>
+
           <li><i>Hydrochloric acid (0.66M) : 1L</i></li>
 +
          <li>Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)</li>
 +
          <li><i>Sodium hydroxide (0.75M) : 200mL</i></li>
 +
          <li>Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)</li>
 
           <li></li>
 
           <li></li>
 
           <li></li>
 
           <li></li>

Revision as of 14:37, 28 August 2015

Titration of phytc acid using Megazyme© Kit
  • Preparation of reagent solutions (not supllied) :
    • Trichloroacetic acid (50% w/v) : 100mL
    • Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)
    • Hydrochloric acid (0.66M) : 1L
    • Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)
    • Sodium hydroxide (0.75M) : 200mL
    • Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)