Difference between revisions of "Team:Paris Bettencourt/Protocols"

Line 38: Line 38:
 
</script>
 
</script>
  
−
<div id="protocolsGallerie">
 
−
  <div class="box">
 
−
    <div class="innerBox yellow">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#AnalyticalDigestion">
 
−
        <p>Analytical digestion</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/5/5e/ParisBettencourt_protocolsPipetting.JPG">
 
−
        <div class="textBox" id="AnalitycalDigestion">
 
−
<b>Analytical digestion protocol</b><br>
 
−
<ul>
 
−
  <li>Prepare the following mix:
 
−
  <ul>
 
−
    <li>2µL 10X Digestion buffer
 
−
    <li>0.5µL Eco31I
 
−
    <li>0.5µL BbsI
 
−
    <li>2µL of DNA (200ng)
 
−
    <li>15µL water
 
−
  </ul>
 
−
  <li>incube 1h at 37°C
 
−
</ul>
 
−
      </div>
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#Annealing">
 
−
        <p>Annealing</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/1/11/ParisBettencourt_protocolsGel.JPG">
 
−
        <div class="textBox" id="Annealing">
 
−
<b>Annealing Protocol</b>
 
−
<ul>
 
−
<li>Phosphorylation of the oligos
 
−
<ul>
 
−
  <li>        5.6μL DNAse/RNAse free water
 
−
  <li> 6.0μL  o15.011 (10µM)
 
−
  <li> 6.0μL  o15.012 (10µM)
 
−
  <li> 2.0μL  10X T4 DNA ligase buffer
 
−
  <li> 0.4μL  T4 PolyNucleotide Kinase
 
−
Total:  20μL
 
  
−
</ul>
 
−
<br/>
 
−
 
−
  <li>incube 30min at 37°C
 
−
  <li>add 1μL of 1M NaCl
 
−
  <li>incube 5min at 95°C
 
−
  <li>let the mix cool down
 
−
  <li>use 2μL of the mix as a 10X solution
 
−
</ul>
 
−
        </div>
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox magenta">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Electroporation">
 
−
        <p>Electroporation</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/2/24/ParisBettencourt_protocolsCentrifuge.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox yellow">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Electrocompetent Cells Preparation">
 
−
        <p>Electrocompetent Cells Preparation</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/d/da/ParisBettencourt_protocolsErlen.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Digestion">
 
−
        <p>Digestion</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/6/6a/ParisBettencourt_protocolsTubesOnIce.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox magenta">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Electrocompetent Cells Preparation for Lactobacillus Plantarum">
 
−
        <p>Electrocompetent Cells Preparation for <i>Lactobacillus plantarum</i></p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/e/ec/ParisBettencourt_protocolsFrozenEnzymes.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox yellow">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat Shock transformation">
 
−
        <p>Heat Shock transformation</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/0/0d/ParisBettencourt_protocolsBanana.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Titration protocols for vitamin A">
 
−
        <p>Titration protocols for vitamin A</p>
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox magenta">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Yeast lysis with NaOH">
 
−
        <p>Yeast lysis with NaOH</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/c/ce/ParisBettencourt_protocolsTubesIncubator.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox yellow">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat shock transformation for yeast">
 
−
        <p>Heat shock transformation for yeast</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/5/5e/ParisBettencourt_protocolsPipetting.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Ligation">
 
−
        <p>Ligation</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/3/34/ParisBettencourt_protocolsPCR.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox magenta">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Titration protocols for vitamin B2 with HPLC">
 
−
        <p>Titration protocols for vitamin B2 with HPLC</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/1/1c/ParisBettencourt_protocolsTubeUnderHood.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox yellow">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Idli receipe">
 
−
        <p>Idli recipe</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/5/55/ParisBettencourt_protocolsIdli.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Miniprep protocol using a QIAGEN kit">
 
−
        <p>Miniprep protocol using a QIAGEN kit</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/e/ec/ParisBettencourt_protocolsQiagen.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/PCR purification">
 
−
        <p>PCR purification</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/2/23/ParisBettencourt_protocolsPipette.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/PCR">
 
−
        <p>PCR</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/3/34/ParisBettencourt_protocolsPCR.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/gel-purification">
 
−
        <p>Gel purification</p>
 
−
        <img src="https://static.igem.org/mediawiki/2015/1/11/ParisBettencourt_protocolsGel.JPG">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
  <div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/yeast-dna-extraction-dneasy-blood-and-tissue-kit">
 
−
        <p>Yeast DNA extraction using DNeasy® Blood & Tissue Kit [QIAGEN]</p>
 
−
        <img src="">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
<div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/titration-acid-phytic">
 
−
        <p>Titration of phytic acid using Megazyme© Kit</p>
 
−
        <img src="">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
</div>
 
−
<div class="box">
 
−
    <div class="innerBox cyan">
 
−
      <a https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Gel_Electrophoresis">
 
−
        <p>Gel Electrophoresis with SYBR safe</p>
 
−
        <img src="">
 
−
      </a>
 
−
    </div>
 
−
  </div>
 
−
</div>
 
−
 
−
<div style="clear: both;"></div>
 
−
 
−
</div>
 
−
</div>
 
  
 
</html>
 
</html>
 
{{Paris_Bettencourt/footer}}
 
{{Paris_Bettencourt/footer}}

Revision as of 08:35, 31 August 2015