Difference between revisions of "Team:Paris Bettencourt/Protocols/PCR"
| Line 13: | Line 13: | ||
<li>2µL primer Reverse (10 µM)</li> | <li>2µL primer Reverse (10 µM)</li> | ||
<li>0.1 to 1 ng of template DNA</li> | <li>0.1 to 1 ng of template DNA</li> | ||
| − | |||
<li>25µL of <i>Life Technologies</i> Phusion High-Fidelity PCR Master Mix with HF Buffer</li> | <li>25µL of <i>Life Technologies</i> Phusion High-Fidelity PCR Master Mix with HF Buffer</li> | ||
| + | <li>complete to 50µL of DNase/RNase-free water</li> | ||
</ul> | </ul> | ||
<li>Launch the PCR using the following mix | <li>Launch the PCR using the following mix | ||
Revision as of 16:04, 1 September 2015
