Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 134: | Line 134: | ||
</ol> | </ol> | ||
<h2>Goal 2</h2> | <h2>Goal 2</h2> | ||
− | < | + | PCR my backbone and my insert |
+ | <h2>Procedure</h2> | ||
<li>PCR1' = Backbone + 068 and 069 primers, before purify</li> | <li>PCR1' = Backbone + 068 and 069 primers, before purify</li> | ||
<li>PCR2' = FINAL + 068 and 069 primers, before purify</li> | <li>PCR2' = FINAL + 068 and 069 primers, before purify</li> | ||
<li>After PCR and after purification : 1'p = 66,5 2'p =90,5 ng/uL</li> | <li>After PCR and after purification : 1'p = 66,5 2'p =90,5 ng/uL</li> | ||
− | <li>The gel migration shows a bad result for PCR 1’ (Linearized pSB1C3) | + | <li>The gel migration shows a bad result for PCR 1’ (Linearized pSB1C3)</li> |
− | </ | + |
Revision as of 09:53, 7 September 2015