Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
Line 136: | Line 136: | ||
PCR my backbone and my insert | PCR my backbone and my insert | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
− | + | <li>PCR1' = Backbone + 068 and 069 primers, before purify</li> | |
− | + | <li>PCR2' = FINAL + 068 and 069 primers, before purify</li> | |
− | + | <li>After PCR and after purification : 1'p = 66,5 2'p =90,5 ng/uL</li> | |
− | + | <li>The gel migration shows a bad result for PCR 1’ (Linearized pSB1C3)</li> |
Revision as of 09:54, 7 September 2015