Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
CeliaFoulon (Talk | contribs) |
CeliaFoulon (Talk | contribs) |
||
Line 152: | Line 152: | ||
While waiting for the cubes results, we are thinking about the next step: finding a media to grow the strains, easy to give to the population and to use afterward. The perfect media would be an edible one, that we could incorporate to the cube, instead of the water.<br> | While waiting for the cubes results, we are thinking about the next step: finding a media to grow the strains, easy to give to the population and to use afterward. The perfect media would be an edible one, that we could incorporate to the cube, instead of the water.<br> | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
+ | We tried 2 different medium: potato juice and potato juice with rice flour.<br> | ||
+ | For the potato juice: | ||
<ul> | <ul> | ||
<li>Heat 3 potatoes in 750ml of water</li> | <li>Heat 3 potatoes in 750ml of water</li> | ||
Line 160: | Line 162: | ||
<li>Inoculate with Sc. M Cherry</li> | <li>Inoculate with Sc. M Cherry</li> | ||
<li>Put in the incubator at 30°C for 2 days</li> | <li>Put in the incubator at 30°C for 2 days</li> | ||
− | < | + | </ul> |
+ | For the mix potato juice with rice flour: | ||
+ | <ul> | ||
+ | <li>Put 5ml of the potatoe water made previously in a culture tube</li> | ||
+ | <li>Inoculate with Sc. M Cherry</li> | ||
+ | <li>Add 20μL of geneticin (100mg/ml)</li> | ||
+ | <li>Add 5ml of rice flour</li> | ||
+ | <li>Put in the incubator at 30°C for 2 days</li> | ||
</ul> | </ul> | ||
Line 175: | Line 184: | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 13th</h1> | <h1 class="date two">August 13th</h1> | ||
− | + | <h2>Results of the media test</h2> | |
+ | Nothing grew on the potato juice media.<br> | ||
+ | We had the idea of adding sugar, to see if it changes something.<br> | ||
+ | The potato juice/rice flour media strongly smells yeasts. We therefore admit the yeast grew in this media but decided that the aspect wasn't very attractive and the smell so so bad that indian population would be disgusted and won't use it. | ||
<br><br> | <br><br> |
Revision as of 15:21, 8 September 2015