Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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<h2>Results of the cube tests</h2> | <h2>Results of the cube tests</h2> | ||
The yeasts survived well: 42%. <br> | The yeasts survived well: 42%. <br> | ||
+ | Seven days later, we plated again cubes from this test, and obtained a survival rate of 21%. | ||
But strangely, the number of cells which are supposed to be Lactococcus, in the plates of M17 glucose with erythromycin, is the same as in the plates with YPD and geneticin, selecting the yeasts.<br> | But strangely, the number of cells which are supposed to be Lactococcus, in the plates of M17 glucose with erythromycin, is the same as in the plates with YPD and geneticin, selecting the yeasts.<br> | ||
Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | Therefore, we checked the production of RFP, since Sc. M Cherry produces RFP but not G1513.<br> | ||
Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | Unfortunately, we came to the conclusion that the cells in the M17 plates were yeasts. Actually, Sc. M Cherry resist to erythromycin and is therefore growing in the plate.<br> | ||
<h2>Goal</h2> | <h2>Goal</h2> | ||
− | We need a chemical that will allow us to select | + | We need a chemical that will allow us to select G1513 and kill Sc. M Cherry.<br> |
− | After some researchs, we found out that cycloheximide could be helpful and ordered it. While waiting for this chemical, we | + | After some researchs, we found out that cycloheximide could be helpful and ordered it. While waiting for this chemical, we can't work with G1513. |
<br><br> | <br><br> | ||
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<br><br> | <br><br> | ||
<h1 class="date two">August 17th</h1> | <h1 class="date two">August 17th</h1> | ||
− | <h2>Procedure/h2> | + | <h2>Procedure</h2> |
Making new cubes with Sc. M Cherry alone, G1513 alone, and a mix of both strains. <br> | Making new cubes with Sc. M Cherry alone, G1513 alone, and a mix of both strains. <br> | ||
Measuring the OD and plating to count the cells | Measuring the OD and plating to count the cells | ||
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<h1 class="date two">August 20th</h1> | <h1 class="date two">August 20th</h1> | ||
<h2>Procedure</h2> | <h2>Procedure</h2> | ||
− | Making more cubes with Sc. M Cherry alone, G1513 alone, and a mix of both. | + | Making more cubes with Sc. M Cherry alone, G1513 alone, and a mix of both.<br> |
− | + | Making more OD correlations. | |
− | + | ||
− | + | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 24th</h1> | <h1 class="date two">August 24th</h1> | ||
+ | <h2>Procedure</h2> | ||
+ | Making more cubes...<br> | ||
+ | <h2>Results of the OD test</h2> | ||
+ | For Sc. M Cherry:<br> | ||
+ | <b>1 OD = 3,1x10^6 cells/mL</b><br> | ||
+ | For G1513:<br> | ||
+ | Nothing... It seems that the colony used was dead so the cubes will not contained any G1513.<br> | ||
+ | <h2>Results of the cube test</h2> | ||
+ | <ul> | ||
+ | <li>Sc. M Cherry alone: 92%</li> | ||
+ | <li>Sc. M Cherry in the mix (but dead G1513): 96%</li> | ||
+ | <li>G1513 alone: Nothing</li> | ||
+ | </ul> | ||
<br><br> | <br><br> | ||
<h1 class="date two">August 25th</h1> | <h1 class="date two">August 25th</h1> | ||
+ | |||
<br><br> | <br><br> |
Revision as of 16:58, 8 September 2015