Difference between revisions of "Team:Exeter/Protocols"
| Line 164: | Line 164: | ||
<a target="_blank" href="https://static.igem.org/mediawiki/2015/7/71/Exeter_S30_cell_free_kit_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/b/b6/Exeter_S30_cell_free.png" style="height:250px"/></a> | <a target="_blank" href="https://static.igem.org/mediawiki/2015/7/71/Exeter_S30_cell_free_kit_SOP.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/b/b6/Exeter_S30_cell_free.png" style="height:250px"/></a> | ||
<a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/9/94/Exeter_solid_agar.png" style="height:250px"/></a> | <a target="_blank" href="https://static.igem.org/mediawiki/2015/3/3d/Exeter_solid_agar_recipe.pdf"><img class="cover" src="https://static.igem.org/mediawiki/2015/9/94/Exeter_solid_agar.png" style="height:250px"/></a> | ||
| + | <a target="_blank" href="https://static.igem.org/mediawiki/2015/0/0e/Exeter_sds_page_sop.png"><img class="cover" src="https://static.igem.org/mediawiki/2015/9/94/Exeter_solid_agar.png" style="height:250px"/></a> | ||
| + | <a target="_blank" href=""><img class="cover" src="https://static.igem.org/mediawiki/2015/9/95/Exeter_Q5_mutagenesis_primers.png" style="height:250px"/></a> | ||
</div> | </div> | ||
<br/> | <br/> | ||
Revision as of 14:26, 13 September 2015
Protocols
Use the mouse or touch screen to scroll through our SOPs. Click on an image to bring up the corresponding protocol.
- E.coli transformation.
- gBlock Prep. and HiFi assembly.
- Competent cells preparation.
- Protein extraction (BugBuster).
- LB agar broth recipe.
- Agarose gel recipe.
- Running gel electrophoresis.
- Using the autoclave.
- DNA restriction digest.
- Thermo scientific FastDigest.
- DNA gel extraction.
- Cell glycerol stocks.
- Promega DNA ligation.
- Qiagen DNA miniprep.
- Liquid cell cultures.
- MgCl2 and CaCl2 recipes.
- Sterile 40% glycerol recipe.
- Qubit for DNA concentration.
- Dried DNA rehydration.
- Promega S30 cell free kit.
- Solid agar recipe for agar plates.