Difference between revisions of "Team:Paris Bettencourt/Project/VitaminB12"
| Line 6: | Line 6: | ||
<h2>Introduction</h2> | <h2>Introduction</h2> | ||
<p>Vitamin B12, or cobalamin, is a family of molecules that are involved in only two chemical reactions in humans. | <p>Vitamin B12, or cobalamin, is a family of molecules that are involved in only two chemical reactions in humans. | ||
| − | However a cobalamin deficiency can lead to | + | However a cobalamin deficiency can lead to problems related to the nervous system and blood formation. |
Diseases related to low blood levels of cobalamin include fatigue, depression, low red blood cells level and even sensory loss and infertility. <br> | Diseases related to low blood levels of cobalamin include fatigue, depression, low red blood cells level and even sensory loss and infertility. <br> | ||
https://books.google.fr/books?id=b9ahvNjPFO8C&lpg=PA245&hl=fr&pg=PA246#v=onepage&q&f=false <br> | https://books.google.fr/books?id=b9ahvNjPFO8C&lpg=PA245&hl=fr&pg=PA246#v=onepage&q&f=false <br> | ||
| Line 13: | Line 13: | ||
<p> | <p> | ||
| − | Its production is extremely complicated. In the 60s and 70s, it took ten years and 72 steps | + | Its production is extremely complicated. In the 60s and 70s, it took ten years and 72 steps for Woodward <i>et al.</i> and Eschemoser <i>et al.</i> to chemically synthesize it.<br> |
http://pac.iupac.org/publications/pac/pdf/1973/pdf/3301x0145.pdf <br> | http://pac.iupac.org/publications/pac/pdf/1973/pdf/3301x0145.pdf <br> | ||
http://www.synarchive.com/syn/71<br> | http://www.synarchive.com/syn/71<br> | ||
Revision as of 18:19, 15 September 2015
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Background
Aims
Results
Cobalamin (vitamin B12) deficiency is widely spread in India, due to diet that is mostly vegetarian. We aimed at introducing a high-level cobalamin producer to the rice batter. Tadaa.
Introduction
Vitamin B12, or cobalamin, is a family of molecules that are involved in only two chemical reactions in humans. However a cobalamin deficiency can lead to problems related to the nervous system and blood formation. Diseases related to low blood levels of cobalamin include fatigue, depression, low red blood cells level and even sensory loss and infertility.
https://books.google.fr/books?id=b9ahvNjPFO8C&lpg=PA245&hl=fr&pg=PA246#v=onepage&q&f=false
http://www.bmj.com/content/349/bmj.g5226.long
Its production is extremely complicated. In the 60s and 70s, it took ten years and 72 steps for Woodward et al. and Eschemoser et al. to chemically synthesize it.
http://pac.iupac.org/publications/pac/pdf/1973/pdf/3301x0145.pdf
http://www.synarchive.com/syn/71
But this synthesis is costly and needs time. This is why cobalamin is produced by bacteria, the only living organisms that can produce it.
Ideas
Express the cobalamin biosynthesis pathway in Lactobacillus plantarum
Make Lactobacillus reuteri express a lot of cobalamin
Metabolic engineering
The pseudo-cobalamin problem
Use Propionibacterium freudenreichii
Advices for other teams
Conclusion
Click here to see our solution!
