Difference between revisions of "Team:Paris Bettencourt/Notebook/Riboswitch"
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Gel order : GR1KB-10-11-12-13-14-15- GR1KB-20-21-22-23-24-25- GR1KB-30-31-32-33-34-35<br> | Gel order : GR1KB-10-11-12-13-14-15- GR1KB-20-21-22-23-24-25- GR1KB-30-31-32-33-34-35<br> | ||
<img src="https://static.igem.org/mediawiki/2015/a/ae/2131.png" width="600px"> | <img src="https://static.igem.org/mediawiki/2015/a/ae/2131.png" width="600px"> | ||
+ | <h2>Goal 2</h2> | ||
+ | Gibson 1 - New PCR | ||
+ | <h2>Procedure</h2> | ||
+ | <style type="text/css"> | ||
+ | .tg {border-collapse:collapse;border-spacing:0;} | ||
+ | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} | ||
+ | </style> | ||
+ | <table class="tg"> | ||
+ | <tr> | ||
+ | <th class="tg-031e"></th> | ||
+ | <th class="tg-031e">B5</th> | ||
+ | <th class="tg-031e">B6</th> | ||
+ | <th class="tg-031e">B7</th> | ||
+ | <th class="tg-031e">I3</th> | ||
+ | <th class="tg-031e">I4</th> | ||
+ | <th class="tg-031e">I5</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Template</td> | ||
+ | <td class="tg-031e">1ng</td> | ||
+ | <td class="tg-031e">3ng</td> | ||
+ | <td class="tg-031e">6ng</td> | ||
+ | <td class="tg-031e">1ng</td> | ||
+ | <td class="tg-031e">3ng</td> | ||
+ | <td class="tg-031e">6ng</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Primers</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | <td class="tg-031e">2uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">MasterMixPhusion</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | <td class="tg-031e">25uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Water</td> | ||
+ | <td class="tg-031e">20 uL</td> | ||
+ | <td class="tg-031e">20 uL</td> | ||
+ | <td class="tg-031e">20 uL</td> | ||
+ | <td class="tg-031e">20 uL</td> | ||
+ | <td class="tg-031e">20 uL</td> | ||
+ | <td class="tg-031e">20 uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">TOT</td> | ||
+ | <td class="tg-031e">50 uL</td> | ||
+ | <td class="tg-031e">50 uL</td> | ||
+ | <td class="tg-031e">50 uL</td> | ||
+ | <td class="tg-031e">50 uL</td> | ||
+ | <td class="tg-031e">50 uL</td> | ||
+ | <td class="tg-031e">50 uL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Primers used</td> | ||
+ | <td class="tg-031e">211-212</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e">209-210</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">T°C annealing</td> | ||
+ | <td class="tg-031e">69°C</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e">66°C</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="tg-031e">Elongation time</td> | ||
+ | <td class="tg-031e">2min</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e">15sec</td> | ||
+ | <td class="tg-031e"></td> | ||
+ | <td class="tg-031e"></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <h2>Results</h2> | ||
+ | Gel order : - - - B5 B6 B7 I4 I5 ( I3 was lost )<br> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/c/c9/7687.png" width="600px"> |
Revision as of 15:06, 16 September 2015