Exeter iGEM Lab Book

Welcome to our lab book. The following pages contain a chronological description of everything we did in the lab – starting on Day 1. Different days contain short descriptions of our experiments and notes, as well as references to the appropriate protocols.

We were not adept at keeping a concise lab book during the first few weeks of iGEM – the descriptions get more organised and detailed as the weeks progress. The first part of our lab book relates to the Interlab Study, and the later parts to our actual project, Ribonostics.

All protocols can be found at the bottom of the page,and there will links throughout. If you have any questions you can email the team or individual members emails can be found on our team page.

04/06/15

Interlab Study - hydrating DNA, transformation into competent DH5α cells, overnight cultures, mini prep.

05/06/15

Digestion of our insert and backbone.

Results of our transformations from our plates.