Difference between revisions of "Team:Paris Bettencourt/Project/Continuity"
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<h2>The ID gene</h2> | <h2>The ID gene</h2> | ||
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− | The quality control of our system is possible thanks to an ID gene, which consists in the fluorescent protein mCherry expressed to low levels. | + | <p>The quality control of our system is possible thanks to an ID gene, which consists in the fluorescent protein mCherry expressed to low levels. We chose this protein because its fluorescence colour is easily distinguishable from the media that will be used for growth, hence a better signal.</p> |
− | + | <p>The aim of this gene is to provide a quick and reliable way to determine whether the strain that will be distributed is the one intended. After growing the micro-organism in a bioreactor, a sample is taken in order to start a new culture from it. We suggest that, while doing so, the sample has to pass a quality check where its fluorescence is measured.</p> | |
− | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Modeling" class="readMore buttonCyan">Click here to learn more about the DIλ | + | <p>If the sample displays fluorescence, the culture is sent for packaging and a new culture can be launched. If the fluorescence in not sufficient, the sample is discarded and a new blister stock of the original strain is used to start the culture.</p> |
+ | <p> | ||
+ | This fluorescence measurement is a good example of real-life use for the DIλ spectrophotometer, a low-budget device that is developed by our neighbours at the Openlab. Heavy development is currently ongoing to make it capable of fluorescence measurements. | ||
+ | </p> | ||
+ | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Modeling" class="readMore buttonCyan">Click here to learn more about the DIλ spectrophotometer</a> | ||
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This project was designed and accomplished by Antoine Vigouroux in consultation with Jason Bland and Ihab Boulas. | This project was designed and accomplished by Antoine Vigouroux in consultation with Jason Bland and Ihab Boulas. | ||
− | Most of the strains (DH5alpha, Top10, NEB turbo, Pir116) were kindly provided by Inserm U1001. Plasmids pFHC2938 and pMEV250 were provided by Jason Bland and Aleksandra | + | Most of the strains (DH5alpha, Top10, NEB turbo, Pir116) were kindly provided by Inserm U1001. Plasmids pFHC2938 and pMEV250 were provided by Jason Bland and Aleksandra Nivina at Didier Mazel's lab at Institut Pasteur. Plasmids pL1F2 and pR6K-shortened were provided by Antoine Decrulle and Ihab Boulas at Inserm U1001. The pIT5-KH vector was provided by Lun Cui at David Bikard's lab at Institut Pasteur. |
Special thanks to all the people who gave me an hand during this project, and all the Paris Bettencourt team for making it so much fun. | Special thanks to all the people who gave me an hand during this project, and all the Paris Bettencourt team for making it so much fun. | ||
</html> | </html> | ||
{{Paris_Bettencourt/footer}} | {{Paris_Bettencourt/footer}} |
Revision as of 22:09, 18 September 2015