Difference between revisions of "Team:Paris Bettencourt/Project/Continuity"
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{{Paris_Bettencourt/header}} | {{Paris_Bettencourt/header}} | ||
{{Paris_Bettencourt/menu}} | {{Paris_Bettencourt/menu}} | ||
− | {{Paris_Bettencourt/ | + | {{Paris_Bettencourt/continuityBanner}} |
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<img src="https://static.igem.org/mediawiki/2015/8/88/PB_growth.png"/> | <img src="https://static.igem.org/mediawiki/2015/8/88/PB_growth.png"/> | ||
− | <p class="caption"> | + | <p class="caption">Our model for decreasing the fitness burden of our organism during the production phase. The cells do not produce any vitamin during the manufacturing process. The vitamin production is triggered before the distribution.</p> |
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<div style="clear:both"></div> | <div style="clear:both"></div> | ||
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<div class="column-right"> | <div class="column-right"> | ||
− | <img src="https://static.igem.org/mediawiki/2015/f/ff/PB_workflow.png"/> | + | <a href="https://static.igem.org/mediawiki/2015/f/ff/PB_workflow.png"> |
+ | <img src="https://static.igem.org/mediawiki/2015/f/ff/PB_workflow.png" width="90%"/> | ||
+ | </a> | ||
+ | <span class="caption">How quality control will be performed. The transfer of the inoculate should be done in sterile conditions in a container that also plays the role of fluorimeter cuvette. This ensures that even low-budget labs will always distribute the right strain to people.</span> | ||
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− | The cells exhibit clear fluorescence (Mann-Whitney test, p-value < 10<sup>-6</sup>), even though it was not visible to the naked eye. | + | The cells exhibit clear fluorescence (Mann-Whitney test, p-value < 10<sup>-6</sup>), even though it was not visible to the naked eye. The mCherry fluorescent protein is therefore a suitable reporter for quality control of the strain. |
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</div> | </div> | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
− | <h2 | + | <h2>Induction of the differentiation</h2> |
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− | < | + | <img src="https://static.igem.org/mediawiki/2015/c/c1/PB_empty.png" /> |
+ | <p class="caption">caption</p></p> | ||
− | + | <img src="https://static.igem.org/mediawiki/2015/d/da/PB_beforeaftercre.png"/> | |
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− | + | <h1>Outlook</h1> | |
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− | <h1 | + | |
<p>link</p> | <p>link</p> | ||
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<h1>Litterature</h1> | <h1>Litterature</h1> | ||
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Most of the strains (DH5alpha, Top10, NEB turbo, Pir116) were kindly provided by Inserm U1001. Plasmids pFHC2938 and pMEV250 were provided by Jason Bland and Aleksandra Nivina at Didier Mazel's lab at Institut Pasteur. Plasmids pL1F2 and pR6K-shortened were provided by Antoine Decrulle and Ihab Boulas at Inserm U1001. The pIT5-KH vector was provided by Lun Cui at David Bikard's lab at Institut Pasteur. | Most of the strains (DH5alpha, Top10, NEB turbo, Pir116) were kindly provided by Inserm U1001. Plasmids pFHC2938 and pMEV250 were provided by Jason Bland and Aleksandra Nivina at Didier Mazel's lab at Institut Pasteur. Plasmids pL1F2 and pR6K-shortened were provided by Antoine Decrulle and Ihab Boulas at Inserm U1001. The pIT5-KH vector was provided by Lun Cui at David Bikard's lab at Institut Pasteur. | ||
− | Special thanks to all the people who gave me an hand during this project, and all the Paris Bettencourt team for making | + | Special thanks to all the people who gave me an hand during this project, and all the Paris Bettencourt team for making this adventure so much fun. |
</html> | </html> | ||
{{Paris_Bettencourt/footer}} | {{Paris_Bettencourt/footer}} |
Revision as of 23:47, 18 September 2015