Difference between revisions of "Team:Aachen/InteractiveTour52"
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− | In parallel to establishing methanol as a carbon source for ''E. coli'', we also enabled it to accumulate remarkable amounts of glycogen. We created and characterized single knockouts of ''glgX'' and ''glgP'' in Escherichia coli BL21 Gold (DE3). This was done with CRISPR/Cas9 genome editing which we found to be a very efficient method for genome modifications. In addition we assembled and characterized a functional glycogen synthesis operon. | + | In parallel to establishing methanol as a carbon source for ''E. coli'', we also enabled it to accumulate remarkable amounts of glycogen. We created and characterized single knockouts of ''glgX'' and ''glgP'' in Escherichia coli BL21 Gold (DE3) and were also able to do a double-knockout of both genes in ''E. coli'' NEB10β. This was done with CRISPR/Cas9 genome editing which we found to be a very efficient method for genome modifications. In addition we assembled and characterized a functional glycogen synthesis operon. |
− | By combining the characterized synthesis operon (''glgCAB'') and the knockout of ''glgP'' in one organism, further enhanced glycogen formation. | + | By combining the characterized synthesis operon (''glgCAB'') and the knockout of ''glgP'' in one organism, we further enhanced glycogen formation. |
Revision as of 01:08, 19 September 2015