These days, the whole bioeconomy is dependent on one valuable resource: sugars. No matter if they are produced from starch or cellulose, they always originate from plants. But while the global demand for carbon sources is growing, the arable land is shrinking and droughts are more frequent.

At iGEM Aachen, we aim to make the bioeconomy independent from plant-derived sugars. We do this by teaching E. coli to use methanol as a carbon source and convert it to glycogen, the bacterial equivalent to starch.

Therefore, we introduce a synthetic pathway, implementing it in vivo for the first time.

For this kind of metabolic engineering research, chemostat cultivation of candidate strains is essential. However, traditional continous cultivations are very costly and not affordable for everyone. To solve this problem, we are developing a do-it-yourself bioreactor with very low culture volume. This bioreactor is accompanied by hardware and software that is cost-effective and user-friendly.

Concering the DIY principle of our modular selfmade bioreactor we established contacts to community labs to discuss about the developement of the European Community Lab Scene.

Regarding biosafety in the lab we tested and discussed our new documentation system with other teams to explore the technology and experiences.

Motivation

When we first started with extensive literature research at the beginning of the project, we came across an interesting paper about an ATP neutral cycle for methanol uptake ^[1] Right from the beginning we saw the great potential of methanol as a carbon source that is currently produced in large amounts but only partially used for fuels and not nearly up to its full potential. At this point we had the base for our project idea. This was further developed by research about other sources of methanol. We investigated the technical fixation of CO₂ from the air and by getting in contact with the company sunfire and found out that methanol can easily produced with this method. But what comes next after methanol uptake and how can we make it available for various industries? The answer is sugars. In the bioeconomy most processes rely on sugar and therefore we decided tu convert the methanol into glycogen, the storage molecule of sugar in bacteria.

References