Difference between revisions of "Team:Paris Bettencourt/Project/VitaminB2"
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<b>Figure 4: </b><i>p15.07 is a derivative plasmid from p15.01 after golden gate assembly of Vitamin B2 pathway. Here RibA and RibT cassettes have P48 synthetic promoter and rest two cassettes RibD, RibE have P25 synthetic promoter.</i><br><br> | <b>Figure 4: </b><i>p15.07 is a derivative plasmid from p15.01 after golden gate assembly of Vitamin B2 pathway. Here RibA and RibT cassettes have P48 synthetic promoter and rest two cassettes RibD, RibE have P25 synthetic promoter.</i><br><br> | ||
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After cloning, cells began to release riboflavin in their surrounding media, suggesting that the expression system was functioning in E. coli as well. <br> | After cloning, cells began to release riboflavin in their surrounding media, suggesting that the expression system was functioning in E. coli as well. <br> | ||
Riboflavin concentration corresponding to the fluorescence (at 565nm) of the supernatant was estimated on a standard curve.<br> | Riboflavin concentration corresponding to the fluorescence (at 565nm) of the supernatant was estimated on a standard curve.<br> |
Revision as of 14:58, 15 October 2015