Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression"
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+ | =Raw lab notebook entries= | ||
+ | {{#calendar: year=2015 | month= may | title = Team:UCLA/Notebook/Recombinant_Expression | query=preload=Template:UCLA}} | ||
+ | {{#calendar: year=2015 | month= jun | title = Team:UCLA/Notebook/Recombinant_Expression | query=preload=Template:UCLA}} | ||
+ | {{#calendar: year=2015 | month= jul | title = Team:UCLA/Notebook/Recombinant_Expression | query=preload=Template:UCLA}} | ||
+ | {{#calendar: year=2015 | month= aug | title = Team:UCLA/Notebook/Recombinant_Expression | query=preload=Template:UCLA}} | ||
+ | {{#calendar: year=2015 | month= sep | title = Team:UCLA/Notebook/Recombinant_Expression | query=preload=Template:UCLA}} | ||
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Week 7 | Week 7 | ||
<summary>05/11 – 05/15</summary> | <summary>05/11 – 05/15</summary> |
Revision as of 23:57, 9 July 2015
Recombinant Silk Functionalization
mm/dd - mm/dd (most recent weeks on top)
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mm/dd: Sample Entry
Today we began cloning our GFP.
- PCR'd off template
- Ran gel
- Restriction digest
- Ligated into backbone
For our ligation, we made the following modifications:
- Tried it with newly bought ligase
- Left reaction overnight instead of 2 hrs
- Vector to insert ratio was 1:5 instead of 1:3
PCR Reaction:
Component | Volume |
5X Q5 Reaction Buffer | 5 |
10 mM dNTPs | 0.5 |
10 uM Forward (primer 3/7) | 1.25 |
10 uM Reverse (primer 8) | 1.25 |
Template (diluted to 1ng/uL) | 0.5 |
Q5 High Fidelity DNA Polymerase | 0.25 |
Nuclease Free Water | 16.25 |
Gel: Lot of bands, all at correct sizes
Contents
Goals
Julian's Goals
- Finish Purification of ABD containing construct
- Create hydrogel with ABD and B.mori silk and see if it retains/binds albumin
Achievements
Julian's Achievements
- Cloned the ABD construct and begun purification methods
What to accomplish next
Julian
- Test albumin binding properties of the ABD silk construct
- test hydrogel formation and albumin retention
Raw lab notebook entries
May | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | 31 |
June | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | 6 | 7 |
8 | 9 | 10 | 11 | 12 | 13 | 14 |
15 | 16 | 17 | 18 | 19 | 20 | 21 |
22 | 23 | 24 | 25 | 26 | 27 | 28 |
29 | 30 |
July | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | ||
6 | 7 | 8 | 9 | 10 | 11 | 12 |
13 | 14 | 15 | 16 | 17 | 18 | 19 |
20 | 21 | 22 | 23 | 24 | 25 | 26 |
27 | 28 | 29 | 30 | 31 |
August | ||||||
M | T | W | T | F | S | S |
1 | 2 | |||||
3 | 4 | 5 | 6 | 7 | 8 | 9 |
10 | 11 | 12 | 13 | 14 | 15 | 16 |
17 | 18 | 19 | 20 | 21 | 22 | 23 |
24 | 25 | 26 | 27 | 28 | 29 | 30 |
31 |
September | ||||||
M | T | W | T | F | S | S |
1 | 2 | 3 | 4 | 5 | 6 | |
7 | 8 | 9 | 10 | 11 | 12 | 13 |
14 | 15 | 16 | 17 | 18 | 19 | 20 |
21 | 22 | 23 | 24 | 25 | 26 | 27 |
28 | 29 | 30 |