Revision as of 13:59, 12 August 2015

Ferment It Yourself

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Transformation results

Transformed product	pKV6	pSIP411	p15.01	p15.02	negative control
	(ery 150µg/mL): 500 colonies, no background	(ery 10µg/mL): no growth (ery 150µg/mL): lawn of bacteria	spreading of 170µL: 41 isolated colonies -> isolation of three transformants: T1, T2 and T3 spreading of 50µL: 3 colonies on a lawny background	(ery 10µg/mL): lawn of bacteria (ery 150µg/mL): lawny background	no growth

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