Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminB12"

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<b>Transformation results</b>
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<table style="width=100%">
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<h3>June</h3>
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  <tr>
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Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?
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    <td><b>Transformed product</b></td>
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<h4>Ideas:</h4>
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    <td><b>pKV6</b></td>
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Lactobacillus reuteri
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    <td><b>pSIP411</b></td>
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    <td><b>p15.01</b></td>
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    <td><b>p15.02</b></td>
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<hr>
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    <td><b>negative control</b></td>
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<h4>July</h4>
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  </tr>
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  <tr>
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    <td>  </td>
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    <td><ul><li>(ery 150µg/mL):
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          <br>500 colonies, no background</ul></td>
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    <td><ul>
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          <li>(ery 10µg/mL):<br>
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          no growth
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          <li>(ery 150µg/mL):<br>
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          lawn of bacteria</ul></td>
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    <td>
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    <ul>
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      <li>spreading of 170µL:<br>
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      41 isolated colonies -> isolation of three transformants: T1, T2 and T3
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      <li>spreading of 50µL:<br>
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      3 colonies on a lawny background
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    </ul>
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    </td>
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    <td><ul>
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          <li>(ery 10µg/mL):<br>
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          lawn of bacteria
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          <li>(ery 150µg/mL):<br>
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          lawny background</ul>
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    </td>
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    <td> no growth </td>
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  </tr>
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</table>
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Revision as of 16:56, 12 August 2015

June

Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?

Ideas:

Lactobacillus reuteri

July