Difference between revisions of "Team:Paris Bettencourt/Notebook/Manufacturing"
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− | <b>Tuesday, Jul. 28</b> | + | <b>Tuesday, Jul. 28</b><br> |
− | First idea: drying the yeasts | + | First idea: drying the yeasts. <br> |
+ | <i>Protocol:</i> <br> | ||
<ul> | <ul> | ||
− | |||
<li>Centrifuge the culture tube</li> | <li>Centrifuge the culture tube</li> | ||
<li>Throw the media away, replace it with a known quantity of osmosed water, vortex, take the OD</li> | <li>Throw the media away, replace it with a known quantity of osmosed water, vortex, take the OD</li> | ||
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<li>Let it dry for 2 hours</li> | <li>Let it dry for 2 hours</li> | ||
<li>Scratch the aluminium to detach the dried yeasts</li> | <li>Scratch the aluminium to detach the dried yeasts</li> | ||
+ | <li>Plate the powder obtained to see if Sc. M Cherry survived </li> | ||
</ul> | </ul> | ||
+ | <i>Results:</i><br> | ||
+ | The aluminium is not a good idea, the yeasts stick to it and it gets destroyed when we scratch. We have to find another drying paper.<br> | ||
− | < | + | <b>Wednesday, Jul. 29</b><br> |
+ | Result of the first OD test:<br> | ||
+ | The 10mL of solution had an OD of 2,557 and 100µL of the 10^-4 dilution grew 359 colonies.<br> | ||
+ | so '''1 OD = 1,4x10^7 cells/mL'''<br> | ||
+ | We launched a similar experience for more repetitions.<br> | ||
<h1>eiuaeuaie</h1> | <h1>eiuaeuaie</h1> |
Revision as of 11:54, 13 August 2015